Transmembrane proteins are capable of passing across a membrane, whereas non-transmembrane proteins can not. For the criteria of selection for C. elegans, the transmembrane and non-transmembrane proteins were separated. The transmembrane proteins were then deleted of the transmembrane spanning segments. The remaining extracellular and intracellular fragments from proteins with three or less transmembrane spanning segments were then put into the pool with the previous non-transmembrane spanning proteins. Each of these pooled fractions were then subjected to a size filter, sequence purge, and a PDB purge.
For more information about the selection of proteins, click here.
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