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JCSG Primer Selection Tool
Target List:
File Format: Gene Name <TAB> Start <TAB> End <NEWLINE>
Sequence FASTA file:
FASTA includes stop codons:
Temperature:
C (62.5)
Tolerance:
C (2.5)
Salt Concentration:
uM (100)
T
m
= 81.5 + 16.6 log [Na
+
] + 41(G + C)/length - 500/length
Min Sequence:
bp (18)
Max Sequence:
bp (35)
Theoretical bp Range:
Forward Primer Restriction Site:
Reverse Primer Restriction Site:
Prepend ATG to segments:
Convert xTG to ATG on full or truncated sequences:
File Format
Excel
Tab Delimited
Operon Form
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