JCSG Target History

		Search JCSG

Target ID:

282020

Target Name:

TM0140

Protein Accession:

TM0140

Local Accession:

TM0140

cDNA Accession:

TM0140

PSCA

PDB:1j5t

Hover over nodes to see experimental data. Click on nodes to go to that point in the data below.

Proposed Target

Active Target

PCR Testing

Amplifer Generation

Orientation

Plasmid Purification

Expression

Protein Purification

Crystallization

Crystal Hit

SDC Receiving

Screening

Data Collection

Integration

Scaling

Truncating

Phasing

Tracing

Refining

Validation

PDB Deposition

Proposed Target

Active Target

PCR Testing

Amplifer Generation

Orientation

Plasmid Purification

Expression

Protein Purification

Crystallization

Crystal Hit

SDC Receiving

Screening

Data Collection

Integration

Scaling

Truncating

Phasing

Tracing

Refining

Validation

PDB Deposition

Status View


Chrono View

Name:	Indole-3-glycerol phosphate synthase (EC 4.1.1.48) (IGPS).	Start Position:	1	End Position:	230
GNF Target Alias:	DL41:MH1-TM0140-tma-230-1-230	Sequence Type:	COMPLETE	Residues:	230
DNA Length:	690	Molecular Weight:	26008.62	Isoelectric Point:	5.46
Epsilon 280:	16960	Gravy Index:	-0.10	Species:	T. maritima
Activated:	4	Alanine Absolute:	22	Alanine Relative:	0.0957
Arginine Absolute:	17	Arginine Relative:	0.0739	Asparagine Absolute:	7
Asparagine Relative:	0.0304	Aspartic Acid Absolute:	17	Aspartic Acid Relative:	0.0739
Cysteine Absolute:	1	Cysteine Relative:	0.0043	Glutamic Acid Absolute:	22
Glutamic Acid Relative:	0.0957	Glutamine Absolute:	3	Glutamine Relative:	0.0130
Glycine Absolute:	9	Glycine Relative:	0.0391	Histidine Absolute:	3
Histidine Relative:	0.0130	Isoleucine Absolute:	22	Isoleucine Relative:	0.0957
Leucine Absolute:	22	Leucine Relative:	0.0957	Lysine Absolute:	17
Lysine Relative:	0.0739	Methionine Absolute:	5	Methionine Relative:	0.0217
Phenylalanine Absolute:	9	Phenylalanine Relative:	0.0391	Proline Absolute:	8
Proline Relative:	0.0348	Serine Absolute:	13	Serine Relative:	0.0565
Threonine Absolute:	8	Threonine Relative:	0.0348	Tryptophan Absolute:	2
Tryptophan Relative:	0.0087	Tyrosine Absolute:	4	Tyrosine Relative:	0.0174
Valine Absolute:	19	Valine Relative:	0.0826	Project:	GNF
Selection Date:	2001-08-10

STAGE: Proposed Target

Plate: 0 Cell: 282020 Date: 2001-08-10 Status: Success

STAGE: Active Target

Plate: 594 Cell: D8 Date: 2002-06-25 Status: Success - Backfilled Previous Location: Plate: 0 Cell: 282020

Primer Plate: 594 Date: 2002-06-25
Primer Well: D8 Date: 2002-06-25

Plate: 616 Cell: D8 Date: 2001-10-09 Status: Success - Backfilled Previous Location: Plate: 0 Cell: 282020

Primer Plate: 616 Date: 2001-10-02
Primer Well: D8 Date: 2001-10-02

Plate: 629 Cell: G3 Date: 2001-08-10 Status: Success - Backfilled Previous Location: Plate: 0 Cell: 282020

Primer Plate: 629 Date: 2001-08-17
Primer Well: G3 Date: 2001-08-17
Sample Name: DL41:MH1-TM0140-tma-230-1-230 Date: 2006-09-16
GNF Project Name: Thermotoga maritima Date: 2006-09-16
GNF Program Name: JCSG Date: 2006-09-16

Plate: 646 Cell: B3 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 0 Cell: 282020

Primer Plate: 646 Date: 2002-03-20
Primer Well: B3 Date: 2002-03-20

STAGE: PCR Testing

Plate: 594 Cell: D8 Date: 2002-06-25 Status: Success - Backfilled Previous Location: Plate: 594 Cell: D8
Plate: 616 Cell: D8 Date: 2001-10-09 Status: Success - Backfilled Previous Location: Plate: 616 Cell: D8
Plate: 629 Cell: G3 Date: 2001-08-10 Status: Success - Backfilled Previous Location: Plate: 629 Cell: G3
Plate: 646 Cell: B3 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 646 Cell: B3

STAGE: Amplifer Generation

Plate: 594 Cell: D8 Date: 2002-06-25 Status: Success - Backfilled Previous Location: Plate: 594 Cell: D8
Plate: 616 Cell: D8 Date: 2001-10-09 Status: Success - Backfilled Previous Location: Plate: 616 Cell: D8
Plate: 629 Cell: G3 Date: 2001-08-10 Status: Success - Backfilled Previous Location: Plate: 629 Cell: G3
Plate: 646 Cell: B3 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 646 Cell: B3

STAGE: Orientation

Plate: 594 Cell: D8 Date: 2002-06-25 Status: Success - Backfilled Previous Location: Plate: 594 Cell: D8
Plate: 616 Cell: D8 Date: 2001-10-09 Status: Success - Backfilled Previous Location: Plate: 616 Cell: D8
Plate: 629 Cell: G3 Date: 2001-08-10 Status: Success - Backfilled Previous Location: Plate: 629 Cell: G3
Plate: 646 Cell: B3 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 646 Cell: B3

STAGE: Plasmid Purification

Plate: 594 (C-0002) Cell: D8 Date: 2002-06-25 Status: Success - Backfilled Previous Location: Plate: 594 (C-0002) Cell: D8

Clone ID: 140 Date: 2002-06-25

Plate: 616 (C-0112) Cell: D8 Date: 2001-10-09 Status: Success - Backfilled Previous Location: Plate: 616 (C-0112) Cell: D8

Clone ID: 5204 Date: 2002-06-25

Plate: 629 (C-0131) Cell: G3 Date: 2001-08-10 Status: Success - Backfilled Previous Location: Plate: 629 (C-0131) Cell: G3

Clone ID: 7059 Date: 2002-06-25
Strain/Vector: DL41 Date: 2006-09-16
Clone Site: MH1 Date: 2006-09-16
Cloned Date: 2001-08-10 Date: 2006-09-16

Plate: 646 (C-0153) Cell: B3 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 646 (C-0153) Cell: B3

Clone ID: 8666 Date: 2002-06-25

STAGE: Expression

Plate: 25130 (E-0344) Cell: C02 Date: 2004-06-09 Status: Success Previous Location: Plate: 629 (C-0131) Cell: G3

Fermentation Date: 2004-06-09 Date: 2006-09-12
GNFuge Date: 2004-06-16 Date: 2006-09-12
Protein ID: 30711 Date: 2004-07-19
Media: Native-TB Date: 2006-09-12

Plate: 29431 (E-0463) Cell: A09 Date: 2005-02-17 Status: Success Previous Location: Plate: 629 (C-0131) Cell: G3

Fermentation Date: 2005-02-17 Date: 2010-03-09
Protein ID: 41762 Date: 2005-02-22
Media: Native-TB Date: 2006-09-12

Plate: 30670 (E-0502) Cell: A07 Date: 2005-04-29 Status: Success Previous Location: Plate: 629 (C-0131) Cell: G3

Fermentation Date: 2005-04-29 Date: 2006-09-12
Protein ID: 45199 Date: 2005-05-04
Media: Native-TB Date: 2006-09-12

Plate: 30670 (E-0502) Cell: B07 Date: 2005-04-29 Status: Success Previous Location: Plate: 629 (C-0131) Cell: G3

Fermentation Date: 2005-04-29 Date: 2006-09-12
Protein ID: 45211 Date: 2005-05-04
Media: Native-TB Date: 2006-09-12

Plate: 30670 (E-0502) Cell: C07 Date: 2005-04-29 Status: Success Previous Location: Plate: 629 (C-0131) Cell: G3

Fermentation Date: 2005-04-29 Date: 2006-09-12
Protein ID: 45223 Date: 2005-05-04
Media: Native-TB Date: 2006-09-12

Plate: 30670 (E-0502) Cell: D07 Date: 2005-04-29 Status: Success Previous Location: Plate: 629 (C-0131) Cell: G3

Fermentation Date: 2005-04-29 Date: 2006-09-12
Protein ID: 45235 Date: 2005-05-04
Media: Native-TB Date: 2006-09-12

Plate: 664 (E-0060) Cell: H3 Date: 2001-10-09 Status: Success - Backfilled Previous Location: Plate: 616 (C-0112) Cell: D8

Fermentation Date: 2001-10-09 Date: 2002-06-25
GNFuge Date: 2001-10-10 Date: 2002-06-25
Protein ID: 4734 Date: 2002-06-25
Media: Native-TB Date: 2003-03-07

Plate: 664 (E-0060) Cell: H4 Date: 2001-10-09 Status: Success - Backfilled Previous Location: Plate: 616 (C-0112) Cell: D8

Fermentation Date: 2001-10-09 Date: 2002-06-25
GNFuge Date: 2001-10-10 Date: 2002-06-25
Protein ID: 4735 Date: 2002-06-25
Media: Native-TB Date: 2003-03-07

Plate: 669 (E-0053) Cell: G3 Date: 2001-08-24 Status: Success - Backfilled Previous Location: Plate: 629 (C-0131) Cell: G3

Fermentation Date: 2001-08-24 Date: 2006-09-12
Protein ID: 4133 Date: 2002-06-25
Media: Native-TB Date: 2006-09-12

Plate: 697 (E-0100) Cell: B12 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 646 (C-0153) Cell: B3

Fermentation Date: 2002-03-27 Date: 2002-06-25
GNFuge Date: 2002-04-01 Date: 2002-06-25
Protein ID: 8272 Date: 2002-06-25
Media: Se-Met Date: 2003-03-07

Plate: 697 (E-0100) Cell: B3 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 646 (C-0153) Cell: B3

Fermentation Date: 2002-03-27 Date: 2002-06-25
GNFuge Date: 2002-04-01 Date: 2002-06-25
Protein ID: 8263 Date: 2002-06-25
Media: Se-Met Date: 2003-03-07

Plate: 697 (E-0100) Cell: B6 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 646 (C-0153) Cell: B3

Fermentation Date: 2002-03-27 Date: 2002-06-25
GNFuge Date: 2002-04-01 Date: 2002-06-25
Protein ID: 8266 Date: 2002-06-25
Media: Se-Met Date: 2003-03-07

Plate: 697 (E-0100) Cell: B9 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 646 (C-0153) Cell: B3

Fermentation Date: 2002-03-27 Date: 2002-06-25
GNFuge Date: 2002-04-01 Date: 2002-06-25
Protein ID: 8269 Date: 2002-06-25
Media: Se-Met Date: 2003-03-07

STAGE: Protein Purification

Plate: 25130 (E-0344) Cell: C02 Date: 2004-07-19 Status: Success Previous Location: Plate: 25130 (E-0344) Cell: C02
Plate: 29431 (E-0463) Cell: A09 Date: 2005-02-22 Status: Success Previous Location: Plate: 29431 (E-0463) Cell: A09

Purified Concentration (mg/mL): 18.0 Date: 2006-09-12

Plate: 30670 (E-0502) Cell: A07 Date: 2005-05-04 Status: Success Previous Location: Plate: 30670 (E-0502) Cell: A07

Purified Concentration (mg/mL): 13.0 Date: 2006-09-12

Plate: 30670 (E-0502) Cell: B07 Date: 2005-05-04 Status: Success Previous Location: Plate: 30670 (E-0502) Cell: B07

Purified Concentration (mg/mL): 16.0 Date: 2006-09-12

Plate: 30670 (E-0502) Cell: C07 Date: 2005-05-04 Status: Success Previous Location: Plate: 30670 (E-0502) Cell: C07
Plate: 30670 (E-0502) Cell: D07 Date: 2005-05-04 Status: Success Previous Location: Plate: 30670 (E-0502) Cell: D07
Plate: 664 (E-0060) Cell: H3 Date: 2001-10-09 Status: Success - Backfilled Previous Location: Plate: 664 (E-0060) Cell: H3

Protein Version: 2 Date: 2001-10-09
Purification Date: 2001-10-09 Date: 2003-08-11

Plate: 664 (E-0060) Cell: H4 Date: 2001-10-09 Status: Success - Backfilled Previous Location: Plate: 664 (E-0060) Cell: H4

Protein Version: 3 Date: 2001-10-09
Purification Date: 2001-10-09 Date: 2003-08-11

Plate: 669 (E-0053) Cell: G3 Date: 2001-08-24 Status: Success - Backfilled Previous Location: Plate: 669 (E-0053) Cell: G3

Protein Version: 1 Date: 2001-08-24
Purification Date: 2001-08-24 Date: 2003-08-11

Plate: 697 (E-0100) Cell: B12 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B12

Protein Version: 7 Date: 2002-03-27
Purification Date: 2002-03-27 Date: 2003-08-11

Plate: 697 (E-0100) Cell: B3 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Protein Version: 4 Date: 2002-03-27
Purification Date: 2002-03-27 Date: 2003-08-11

Plate: 697 (E-0100) Cell: B6 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B6

Protein Version: 5 Date: 2002-03-27
Purification Date: 2002-03-27 Date: 2003-08-11

Plate: 697 (E-0100) Cell: B9 Date: 2002-03-27 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B9

Protein Version: 6 Date: 2002-03-27
Purification Date: 2002-03-27 Date: 2003-08-11

STAGE: Crystallization

Plate: 1645 (C0018644) Cell: XT Date: 2001-08-29 Status: Success - Backfilled Previous Location: Plate: 669 (E-0053) Cell: G3

Screen Type: Sparse Matrix Date: 2006-09-12
Setup Date: 2001-08-29 Date: 2006-09-12
Setup Temp: 20 Date: 2006-09-12
Crystal Conditions: Hampton CS I/II Date: 2006-09-12
Drop Volume (nl): 50.0 Date: 2006-09-12
Precipitate Volume (nl): 50 Date: 2006-09-12
Reservoir Volume (ul): 75.0 Date: 2006-09-12

Plate: 1646 (C0018155) Cell: XT Date: 2001-08-29 Status: Success - Backfilled Previous Location: Plate: 669 (E-0053) Cell: G3

Screen Type: Coarse Date: 2003-07-03
Setup Date: 2001-08-29 Date: 2002-12-09
Setup Temp: 20 Date: 2002-12-09
Crystal Conditions: Wizard I/II Date: 2002-12-09

Plate: 1647 (C0018354) Cell: XT Date: 2001-08-29 Status: Success - Backfilled Previous Location: Plate: 669 (E-0053) Cell: G3

Screen Type: Grid Date: 2006-09-12
Setup Date: 2001-08-29 Date: 2006-09-12
Setup Temp: 20 Date: 2006-09-12
Crystal Conditions: Hampton Quad Grid Date: 2006-09-12
Drop Volume (nl): 50.0 Date: 2006-09-12
Precipitate Volume (nl): 50 Date: 2006-09-12
Reservoir Volume (ul): 75.0 Date: 2006-09-12

Plate: 1648 (C0018473) Cell: XT Date: 2001-08-29 Status: Success - Backfilled Previous Location: Plate: 669 (E-0053) Cell: G3

Screen Type: Sparse Matrix Date: 2006-09-12
Setup Date: 2001-08-29 Date: 2006-09-12
Setup Temp: 20 Date: 2006-09-12
Crystal Conditions: Hampton Cryo I/PEG Ion Date: 2006-09-12
Drop Volume (nl): 50.0 Date: 2006-09-12
Precipitate Volume (nl): 50 Date: 2006-09-12
Reservoir Volume (ul): 75.0 Date: 2006-09-12

Plate: 1649 (C0017734) Cell: XT Date: 2001-08-29 Status: Success - Backfilled Previous Location: Plate: 669 (E-0053) Cell: G3

Screen Type: Sparse Matrix Date: 2006-09-12
Setup Date: 2001-08-29 Date: 2006-09-12
Setup Temp: 20 Date: 2006-09-12
Crystal Conditions: Wizard Cryo I/II Date: 2006-09-12
Drop Volume (nl): 50.0 Date: 2006-09-12
Precipitate Volume (nl): 50 Date: 2006-09-12
Reservoir Volume (ul): 75.0 Date: 2006-09-12

Plate: 4347 (C0042430) Cell: XT Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Screen Type: Coarse Date: 2003-07-03
Setup Date: 2002-04-05 Date: 2002-12-09
Setup Temp: 4 Date: 2002-12-09
Crystal Conditions: Hampton CS I/II Date: 2002-12-09

Plate: 4348 (C0042310) Cell: XT Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Setup Date: 2002-04-05 Date: 2002-12-09
Setup Temp: 4 Date: 2002-12-09
Crystal Conditions: Wizard I/II Date: 2002-12-09

Plate: 4349 (C0041288) Cell: XT Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Screen Type: Coarse Date: 2003-07-03
Setup Date: 2002-04-05 Date: 2002-12-09
Setup Temp: 4 Date: 2002-12-09
Crystal Conditions: Wizard Cryo I/II Date: 2002-12-09

Plate: 4350 (C0041188) Cell: XT Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Screen Type: Coarse Date: 2003-07-03
Setup Date: 2002-04-05 Date: 2002-12-09
Setup Temp: 4 Date: 2002-12-09
Crystal Conditions: Hampton Cryo/PEG Ion Date: 2002-12-09

Plate: 4351 (C0041896) Cell: XT Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Screen Type: Coarse Date: 2003-07-03
Setup Date: 2002-04-05 Date: 2002-12-09
Setup Temp: 4 Date: 2002-12-09
Crystal Conditions: Hampton Quad Grid Date: 2002-12-09

Plate: 4352 (C0041795) Cell: XT Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Screen Type: Coarse Date: 2003-07-03
Setup Date: 2002-04-05 Date: 2002-12-09
Setup Temp: 20 Date: 2002-12-09
Crystal Conditions: Hampton CS I/II Date: 2002-12-09

Plate: 4353 (C0041661) Cell: XT Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Setup Date: 2002-04-05 Date: 2002-12-09
Setup Temp: 20 Date: 2002-12-09
Crystal Conditions: Wizard I/II Date: 2002-12-09

Plate: 4354 (C0041303) Cell: XT Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Screen Type: Coarse Date: 2003-07-03
Setup Date: 2002-04-05 Date: 2002-12-09
Setup Temp: 20 Date: 2002-12-09
Crystal Conditions: Wizard Cryo I/II Date: 2002-12-09

Plate: 4355 (C0040940) Cell: XT Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Setup Date: 2002-04-05 Date: 2002-12-09
Setup Temp: 20 Date: 2002-12-09
Crystal Conditions: Hampton Cryo/PEG Ion Date: 2002-12-09

Plate: 4356 (C0041853) Cell: XT Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 697 (E-0100) Cell: B3

Screen Type: Coarse Date: 2003-07-03
Setup Date: 2002-04-05 Date: 2002-12-09
Setup Temp: 20 Date: 2002-12-09
Crystal Conditions: Hampton Quad Grid Date: 2002-12-09

STAGE: Crystal Hit

Plate: 1645 (C0018644) Cell: G09 Date: 2001-08-29 Status: Success - Backfilled Previous Location: Plate: 1645 (C0018644) Cell: XT

Setup Date: 2001-08-29 Date: 2004-07-22
Screen Date: 2001-09-26 Date: 2004-07-22
Screen Conditions: Hampton CS I/II G09: 3.4M 1,6-Hexanediol, 0.2M MgCl2, 0.1M TRIS pH 8.5 Date: 2004-07-22

Plate: 1646 (C0018155) Cell: E01 Date: 2001-08-29 Status: Success - Backfilled Previous Location: Plate: 1646 (C0018155) Cell: XT

Setup Date: 2001-08-29 Date: 2004-07-22
Screen Date: 2001-09-26 Date: 2004-07-22

Plate: 1648 (C0018473) Cell: D05 Date: 2001-08-29 Status: Success - Backfilled Previous Location: Plate: 1648 (C0018473) Cell: XT

Setup Date: 2001-08-29 Date: 2004-07-22
Screen Date: 2001-09-26 Date: 2004-07-22
Screen Conditions: Hampton Cryo I/PEG Ion D05: 0.2M MgCl2, 30.0% PEG-400, 0.1M HEPES pH 7.5 Date: 2004-07-22

Plate: 1649 (C0017734) Cell: H12 Date: 2001-08-29 Status: Success - Backfilled Previous Location: Plate: 1649 (C0017734) Cell: XT

Setup Date: 2001-08-29 Date: 2004-07-22
Screen Date: 2001-09-26 Date: 2004-07-22
Screen Conditions: Wizard Cryo I/II H12: 0.2M NaCl, 50.0% PEG-200, 0.1M Phosphate Citrate pH 4.2 Date: 2004-07-22

Plate: 4347 (C0042430) Cell: A04 Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 4347 (C0042430) Cell: XT

Setup Date: 2002-04-05 Date: 2002-10-26
Screen Date: 2002-05-09 Date: 2002-10-26
Screen Conditions: H1 #04: 2M Ammonium sulfate, 0.1M Tris hydrochloride pH 8.5, final pH 8.5 Date: 2003-07-03

Plate: 4347 (C0042430) Cell: A09 Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 4347 (C0042430) Cell: XT

Setup Date: 2002-04-05 Date: 2002-10-26
Screen Date: 2002-05-09 Date: 2002-10-26
Screen Conditions: H2 #03: 25% ethylene glycol Date: 2003-07-03

Plate: 4349 (C0041288) Cell: A01 Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 4349 (C0041288) Cell: XT

Setup Date: 2002-04-05 Date: 2002-10-26
Screen Date: 2002-05-09 Date: 2002-10-26
Screen Conditions: W1cryo #01: 40% MPD, 0.1M Phosphate-citrate pH 4.2, final pH 5 Date: 2003-07-17

Plate: 4350 (C0041188) Cell: F02 Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 4350 (C0041188) Cell: XT

Setup Date: 2002-04-05 Date: 2002-10-26
Screen Date: 2002-05-09 Date: 2002-10-26
Screen Conditions: H1cryo #32: 1.5M ammonium sulfate, 25% glycerol anhydrous Date: 2003-07-03

Plate: 4351 (C0041896) Cell: F07 Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 4351 (C0041896) Cell: XT

Setup Date: 2002-04-05 Date: 2002-10-26
Screen Date: 2002-05-09 Date: 2002-10-26
Screen Conditions: P6K/LiCl #07: 10% PEG-6000, 0.1M citric acid pH 4.0, 1M LiCl, final pH 4 Date: 2003-07-03

Plate: 4352 (C0041795) Cell: B09 Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 4352 (C0041795) Cell: XT

Setup Date: 2002-04-05 Date: 2002-10-26
Screen Date: 2002-05-09 Date: 2002-10-26
Screen Conditions: H2 #09: 2M sodium chloride, 0.1M sodium acetate trihydrate pH 4.6, final pH 4.6 Date: 2003-07-03

Plate: 4352 (C0041795) Cell: H12 Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 4352 (C0041795) Cell: XT

Setup Date: 2002-04-05 Date: 2002-10-26
Screen Date: 2002-05-09 Date: 2002-10-26
Screen Conditions: H2 #48: 2% dioxane, 10% PEG-20000, 0.1M bicine pH 9.0, final pH 9 Date: 2003-07-03

Plate: 4354 (C0041303) Cell: B02 Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 4354 (C0041303) Cell: XT

Setup Date: 2002-04-05 Date: 2002-10-26
Screen Date: 2002-05-09 Date: 2002-10-26
Screen Conditions: W1cryo #08: 35% 2-ethoxyethanol, 0.1M Cacodylate pH 6.5, final pH 6.9 Date: 2003-07-17

Plate: 4356 (C0041853) Cell: B04 Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 4356 (C0041853) Cell: XT

Setup Date: 2002-04-05 Date: 2002-10-26
Screen Date: 2002-04-15 Date: 2002-10-26
Screen Conditions: AmSO4 #10: 1.6M ammonium sulfate, 0.1M HEPES pH 7.0, final pH 7 Date: 2003-07-03

Plate: 4356 (C0041853) Cell: C12 Date: 2002-04-05 Status: Success - Backfilled Previous Location: Plate: 4356 (C0041853) Cell: XT

Setup Date: 2002-04-05 Date: 2002-10-26
Screen Date: 2002-04-15 Date: 2002-10-26
Screen Conditions: P6K #18: 20% PEG-6000, 0.1M bicine pH 9.0, final pH 9 Date: 2003-07-03

STAGE: SDC Receiving

Dewar: 7703 (Chico-04/23/2002) Cane: C16 (C16Chico-04/23/2002) Cell: P1 Date: 2002-04-23 Status: Success - Backfilled Previous Location: Plate: 4356 (C0041853) Cell: C12

Shipment ID: 18 Date: 2002-11-13

Vial ID: 562 Date: 2002-11-07
Cryo Conditions: 25%glyc Date: 2002-11-07
Crystal Comments: nice Date: 2002-11-07
Ship Date: 2002-04-23 Date: 2002-11-07
Crystal Id: 1230 Date: 2002-11-07
Stop Flag: 0 Date: 2003-10-02
SDC Stage: 2500 Date: 2004-05-18
Mount Date: 2002-04-23 Date: 2002-11-07

Dewar: 7703 (Chico-04/23/2002) Cane: C16 (C16Chico-04/23/2002) Cell: P2 Date: 2002-04-23 Status: Success - Backfilled Previous Location: Plate: 4356 (C0041853) Cell: C12

Shipment ID: 18 Date: 2002-11-13

Vial ID: 563 Date: 2002-11-07
Cryo Conditions: 25%glyc Date: 2002-11-07
Crystal Comments: nice Date: 2002-11-07
Ship Date: 2002-04-23 Date: 2002-11-07
Crystal Id: 1231 Date: 2002-11-07
Stop Flag: 0 Date: 2003-10-02
SDC Stage: 2500 Date: 2004-05-18
Mount Date: 2002-04-23 Date: 2002-11-07

Dewar: 6837 (Zeppo-10/11/2001) Cane: 2 (C2Zeppo-10/11/2001) Cell: P1 Date: 2001-10-11 Status: Success - Backfilled Previous Location: Plate: 1649 (C0017734) Cell: H12

Shipment ID: 1 Date: 2002-11-13

Cryo Conditions: no Date: 2002-11-07
Ship Date: 2001-10-11 Date: 2002-11-07
Crystal Id: 340 Date: 2002-11-07
Stop Flag: 0 Date: 2004-02-20
SDC Stage: 2900 Date: 2004-05-18
Mount Date: 2001-10-11 Date: 2002-11-07

STAGE: Screening

Dewar: 7703 (Chico-04/23/2002) Cane: C16 (C16Chico-04/23/2002) Cell: P1 Date: 2002-06-12 Status: JCSG1 Success

Xtal Quality: 0 Date: 2004-09-10
Beamline: SSRL BL11-1 Date: 2004-09-10
Robot Y or N: 1 Date: 2004-09-10
Screen Date: 2002-06-12 Date: 2004-09-10

Dewar: 7703 (Chico-04/23/2002) Cane: C16 (C16Chico-04/23/2002) Cell: P2 Date: 2002-06-12 Status: JCSG1 Success

Xtal Quality: 0 Date: 2004-09-10
Beamline: SSRL BL11-1 Date: 2004-09-10
Robot Y or N: 1 Date: 2004-09-10
Screen Date: 2002-06-12 Date: 2004-09-10

Dewar: 6837 (Zeppo-10/11/2001) Cane: 2 (C2Zeppo-10/11/2001) Cell: P1 Date: 2001-11-20 Status: Success

Xtal Quality: 7 Date: 2004-09-10
Ice Rings: 0 Date: 2004-09-10
Diffraction Quality: 6 Date: 2004-09-10
Single Xtal: 1 Date: 2004-09-10
Spot Shape: 10 Date: 2004-09-10
Maximum Resolution: 3.8 Date: 2004-09-10
Beamline: SSRL BL11-1 Date: 2004-09-10
Robot Y or N: 0 Date: 2004-09-10
Screen Date: 2001-11-20 Date: 2004-09-10

STAGE: Data Collection

Collection: 14635 Cell: 1 Date: 2001-11-20 Status: Success - Backfilled Previous Location: Dewar: 6837 (Zeppo-10/11/2001) Cane: 2 (C2Zeppo-10/11/2001) Cell: P1

CollectData.xml: U--jcsg-xtal_process5-TM0140-340-bic-CollectData.xml Date: 2003-06-12
Beamline.xml: U--jcsg-xtal_process5-TM0140-340-bic-beamline.xml Date: 2003-06-12
Notes file: U--jcsg-xtal_process5-TM0140-340-bic-notes.txt Date: 2003-06-12
Beamline: BL11-1 Date: 2003-06-12
ExperimentType: X-Ray Date: 2003-06-12
Program name: Blu-Ice Date: 2003-06-12
Program version: 3.0 Date: 2003-06-12
Synchrotron: SSRL Date: 2003-06-12
Detector name: Q315 Date: 2003-06-12
Detector type: CCD Date: 2003-06-12
Detector serial: 902 Date: 2003-06-12
Detector manufacturer: ADSC Date: 2003-06-12
BeamlineInfoURL: http://biosync.sdsc.edu/ssrl/BL11-1.html Date: 2003-06-12
MOSFLM_DETECTOR_PARAMETERS: FINDSPOTS YOFFSET 0.0 LIMITS XMIN 0 XMAX 315 YMIN 0 YMAX 315 XSCAN 157.5 YSCAN 157.5 SCANNER ADSC GAIN 0.23 ADCOFFSET 40 NULLPIX 0 OVERLOAD CUTOFF 65534 PROFILE OPTIMIZE CUTOFF 65535 DISTORTION YSCALE 1.0000 TILT 0 TWIST 0 Date: 2003-06-12
MOSFLM_BEAMLINE_PARAMETERS: SYNCHROTRON POLARIZATION 0.92 DIVERGENCE 0.250 0.030 DISPERSION 0.00020 Date: 2003-06-12
DC Date: 2001-11-20 Date: 2003-06-12
Performed By: Ashley Deacon Date: 2003-06-12
Temperature (K): 100 Date: 2003-06-12
Beamline: BL11-1 Date: 2003-06-12
ImageDirectory: /data/jcsg/crystals/TM0140/11_20_01/1c2p1 Date: 2003-06-12
Description: l1s1 Date: 2003-06-12
Wavelength: 0.975913 Date: 2003-06-12
DetectorMode: undefined Date: 2003-06-12
Distance: 449.987310 Date: 2003-06-12
BeamCenter X: 157.500000 Date: 2003-06-12
BeamCenter Y: 157.500000 Date: 2003-06-12
ImagePrefix: 1c2p1_1 Date: 2003-06-12
ImageSuffix: img Date: 2003-06-12
PhiDelta: 1.500000 Date: 2003-06-12
PhiStart: 279.000000 Date: 2003-06-12
FrameNumber start: 1 Date: 2003-06-12
FrameNumber end: 60 Date: 2003-06-12
HPSS Backup Directory: /data/jcsg/crystals/TM0140/11_20_01/1c2p1 Date: 2005-03-22

STAGE: Integration

Cane: 28502 Cell: P1 Date: 2002-04-15 Status: Success - Backfilled Previous Location: Collection: 14635 Cell: 1

STAGE: Scaling

Cane: 28502 Cell: P1 Date: 2002-04-15 Status: Success - Backfilled Previous Location: Cane: 28502 Cell: P1

Cell A: 122.8 Date: 2003-08-12
Cell B: 122.8 Date: 2003-08-12
Cell C: 85.6 Date: 2003-08-12
Angle Alpha: 90.0 Date: 2003-08-12
Angle Beta: 90.0 Date: 2003-08-12
Angle Gamma: 90.0 Date: 2003-08-12

STAGE: Truncating

Cane: 28502 Cell: P1 Date: 2002-04-15 Status: Success - Backfilled Previous Location: Cane: 28502 Cell: P1

STAGE: Phasing

Cane: 28502 Cell: P1 Date: 2002-04-15 Status: Success - Backfilled Previous Location: Cane: 28502 Cell: P1

Performed By: 93 Date: 2005-05-13
Solution Type: MR Date: 2004-09-14
Reference PDB ids: 1igs Date: 2006-03-22
Phasing Date: 2002-04-15 Date: 2006-03-30

STAGE: Tracing

Cane: 28502 Cell: P1 Date: 2002-05-23 Status: Success - Backfilled Previous Location: Cane: 28502 Cell: P1

STAGE: Refining

Cane: 28502 Cell: P1 Date: 2002-05-23 Status: Success - Backfilled Previous Location: Cane: 28502 Cell: P1

Performed By: 56 Date: 2006-03-22
RESOLUTION RANGE HIGH:: 2.7 Date: 2003-08-12
SPACE GROUP: I4 Date: 2003-08-12
Refinement Date : 2002-05-23 Date: 2006-03-30

STAGE: Validation

Cane: 28502 Cell: P1 Date: 2002-07-03 Status: Success - Backfilled Previous Location: Cane: 28502 Cell: P1

STAGE: PDB Deposition

Cane: 28502 Cell: P1 Date: 2002-07-03 Status: Success - Backfilled Previous Location: Cane: 28502 Cell: P1

PDB ID: 1j5t Date: 2002-12-05
RCSB ID: rcsb00164 Date: 2006-03-22
PDB File: 1j5t.pdb Date: 2006-12-07
Deposition Date: 2002-07-03 Date: 2002-12-05


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