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Target ID:

283301

Target Name:

TM1442

Protein Accession:

TM1442

Local Accession:

TM1442

cDNA Accession:

TM1442

PSCA

PDB:1t6r

PDB:1sbo

Hover over nodes to see experimental data. Click on nodes to go to that point in the data below.

Proposed Target     Active Target     PCR Testing     Amplifer Generation     Orientation     Plasmid Purification     Expression     Protein Purification     NMR Structure     Refining     Validation     PDB Deposition     Proposed Target   Active Target   PCR Testing   Amplifer Generation   Orientation   Plasmid Purification   Expression   Protein Purification   NMR Structure   Refining   Validation   PDB Deposition

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Name:	Putative anti-sigma factor antagonist TM1442.		Start Position:	1		End Position:	110
GNF Target Alias:	DL41:MH1-TM1442-tma-110-1-110		Sequence Type:	COMPLETE		Residues:	110
DNA Length:	330		Molecular Weight:	12299.53		Isoelectric Point:	5.81
Epsilon 280:	2980		Gravy Index:	-0.10		Species:	T. maritima
Activated:	2		Alanine Absolute:	5		Alanine Relative:	0.0455
Arginine Absolute:	3		Arginine Relative:	0.0273		Asparagine Absolute:	5
Asparagine Relative:	0.0455		Aspartic Acid Absolute:	10		Aspartic Acid Relative:	0.0909
Cysteine Absolute:	0		Cysteine Relative:	0.0000		Glutamic Acid Absolute:	7
Glutamic Acid Relative:	0.0636		Glutamine Absolute:	3		Glutamine Relative:	0.0273
Glycine Absolute:	4		Glycine Relative:	0.0364		Histidine Absolute:	1
Histidine Relative:	0.0091		Isoleucine Absolute:	12		Isoleucine Relative:	0.1091
Leucine Absolute:	14		Leucine Relative:	0.1273		Lysine Absolute:	13
Lysine Relative:	0.1182		Methionine Absolute:	2		Methionine Relative:	0.0182
Phenylalanine Absolute:	3		Phenylalanine Relative:	0.0273		Proline Absolute:	0
Proline Relative:	0.0000		Serine Absolute:	12		Serine Relative:	0.1091
Threonine Absolute:	6		Threonine Relative:	0.0545		Tryptophan Absolute:	0
Tryptophan Relative:	0.0000		Tyrosine Absolute:	2		Tyrosine Relative:	0.0182
Valine Absolute:	8		Valine Relative:	0.0727		Project:	GNF
Selection Date:	2001-08-10

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STAGE: Proposed Target

• Plate: 0 Cell: 283301 Date: 2001-08-10 Status: Success

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STAGE: Active Target

• Plate: 642 Cell: D5 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 0 Cell: 283301  
• Primer Plate: 642 Date: 2001-12-12
• Primer Well: D5 Date: 2001-12-12
• Sample Name: DL41:MH1-TM1442-tma-110-1-110 Date: 2006-09-16
• GNF Project Name: Thermotoga maritima Date: 2006-09-16
• GNF Program Name: JCSG Date: 2006-09-16
• Plate: 644 Cell: F2 Date: 2001-10-11 Status: Success - Backfilled      Previous Location: Plate: 0 Cell: 283301  
• Primer Plate: 644 Date: 2001-10-04
• Primer Well: F2 Date: 2001-10-04

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STAGE: PCR Testing

• Plate: 642 Cell: D5 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 642 Cell: D5  
• Plate: 644 Cell: F2 Date: 2001-10-11 Status: Success - Backfilled      Previous Location: Plate: 644 Cell: F2  

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STAGE: Amplifer Generation

• Plate: 642 Cell: D5 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 642 Cell: D5  
• Plate: 644 Cell: F2 Date: 2001-10-11 Status: Success - Backfilled      Previous Location: Plate: 644 Cell: F2  

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STAGE: Orientation

• Plate: 642 Cell: D5 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 642 Cell: D5  
• Plate: 644 Cell: F2 Date: 2001-10-11 Status: Success - Backfilled      Previous Location: Plate: 644 Cell: F2  

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STAGE: Plasmid Purification

• Plate: 642 (C-0144) Cell: D5 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 642 (C-0144) Cell: D5  
• Clone ID: 8247 Date: 2002-06-25
• Strain/Vector: DL41 Date: 2006-09-16
• Clone Site: MH1 Date: 2006-09-16
• Cloned Date: 2001-08-10 Date: 2006-09-16
• Plate: 644 (C-0147) Cell: F2 Date: 2001-10-11 Status: Success - Backfilled      Previous Location: Plate: 644 (C-0147) Cell: F2  
• Clone ID: 8525 Date: 2002-06-25

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STAGE: Expression

• Plate: 694 (E-0082) Cell: D5 Date: 2001-10-08 Status: Success - Backfilled      Previous Location: Plate: 642 (C-0144) Cell: D5  
• Fermentation Date: 2001-10-08 Date: 2006-09-12
• Protein ID: 6722 Date: 2002-06-25
• Media: Native-TB Date: 2006-09-12
• Plate: 695 (E-0062) Cell: F2 Date: 2001-10-11 Status: Success - Backfilled      Previous Location: Plate: 644 (C-0147) Cell: F2  
• Fermentation Date: 2001-10-11 Date: 2002-06-25
• GNFuge Date: 2001-10-15 Date: 2002-06-25
• Protein ID: 4899 Date: 2002-06-25
• Media: Native-TB Date: 2003-03-07
• Plate: 8454 (E-0145) Cell: F2 Date: 2002-08-21 Status: Success - Backfilled      Previous Location: Plate: 642 (C-0144) Cell: D5  
• Fermentation Date: 2002-08-21 Date: 2006-09-12
• Protein ID: 11704 Date: 2002-09-06
• Media: Native-TB Date: 2006-09-12
• Plate: 8454 (E-0145) Cell: F3 Date: 2002-08-21 Status: Success - Backfilled      Previous Location: Plate: 642 (C-0144) Cell: D5  
• Fermentation Date: 2002-08-21 Date: 2006-09-12
• Protein ID: 11705 Date: 2002-09-06
• Media: Native-TB Date: 2006-09-12
• Plate: 8454 (E-0145) Cell: F4 Date: 2002-08-21 Status: Success - Backfilled      Previous Location: Plate: 642 (C-0144) Cell: D5  
• Fermentation Date: 2002-08-21 Date: 2006-09-12
• Protein ID: 11706 Date: 2002-09-06
• Media: Native-TB Date: 2006-09-12

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STAGE: Protein Purification

• Plate: 694 (E-0082) Cell: D5 Date: 2001-12-19 Status: Success - Backfilled      Previous Location: Plate: 694 (E-0082) Cell: D5  
• Protein Version: 2 Date: 2001-12-19
• Purification Date: 2001-12-19 Date: 2003-08-11
• Plate: 695 (E-0062) Cell: F2 Date: 2001-10-11 Status: Success - Backfilled      Previous Location: Plate: 695 (E-0062) Cell: F2  
• Protein Version: 1 Date: 2001-10-11
• Purification Date: 2001-10-11 Date: 2003-08-11
• Plate: 8454 (E-0145) Cell: F2 Date: 2002-08-21 Status: Success - Backfilled      Previous Location: Plate: 8454 (E-0145) Cell: F2  
• Protein Version: 3 Date: 2002-08-21
• Purification Date: 2002-08-21 Date: 2003-08-11
• Plate: 8454 (E-0145) Cell: F3 Date: 2002-08-21 Status: Success - Backfilled      Previous Location: Plate: 8454 (E-0145) Cell: F3  
• Protein Version: 4 Date: 2002-08-21
• Purification Date: 2002-08-21 Date: 2003-08-11
• Plate: 8454 (E-0145) Cell: F4 Date: 2002-08-21 Status: Success - Backfilled      Previous Location: Plate: 8454 (E-0145) Cell: F4  
• Protein Version: 5 Date: 2002-08-21
• Purification Date: 2002-08-21 Date: 2003-08-11

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STAGE: NMR Structure

• Plate: 15678 Cell: 999 Date: 2003-07-31 Status: Success - Backfilled      Previous Location: Plate: 8454 (E-0145) Cell: F3  
• Solver: Touraj Etezadi, Torsten Herrmann Date: 2003-04-15
• Collection: 42301 Cell: 999 Date: 2004-05-07 Status: Success      Previous Location: Plate: 8454 (E-0145) Cell: F3  
• Solver: T. Etezady-Esfarjani, W. Placzek, T. Herrmann Date: 2006-11-22

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STAGE: Refining

• Collection: 22407 Cell: 999 Date: 2003-07-31 Status: Success - Backfilled      Previous Location: Plate: 15678 Cell: 999  
• Refinement Date : 2003-07-31 Date: 2006-04-10
• Collection: 42302 Cell: 999 Date: 2004-05-07 Status: Success      Previous Location: Collection: 42301 Cell: 999  

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STAGE: Validation

• Collection: 22408 Cell: 999 Date: 2004-02-10 Status: Success - Backfilled      Previous Location: Collection: 22407 Cell: 999  
• Collection: 42303 Cell: 999 Date: 2004-05-07 Status: Success      Previous Location: Collection: 42302 Cell: 999  

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STAGE: PDB Deposition

• Collection: 22408 Cell: 999 Date: 2004-02-10 Status: Success      Previous Location: Collection: 22408 Cell: 999  
• PDB ID: 1sbo Date: 2006-10-20
• RCSB ID: RCSB021581 Date: 2006-10-20
• PDB File: C--Documents-and-Settings-amorse-Desktop-1sbo.pdb Date: 2006-10-20
• Deposition Date: 2004-02-10 Date: 2006-10-20
• Collection: 42305 Cell: 1 Date: 2004-05-07 Status: Success      Previous Location: Collection: 42303 Cell: 999  
• PDB ID: 1t6r Date: 2006-11-22
• RCSB ID: RCSB022382 Date: 2006-11-22
• PDB File: C--Documents-and-Settings-amorse-Desktop-1t6r.pdb Date: 2006-11-22
• Deposition Date: 2004-05-07 Date: 2006-11-22

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