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Target ID:

283349

Target Name:

TM1492

Protein Accession:

TM1492

Local Accession:

TM1492

cDNA Accession:

TM1492

PSCA

PDB:1r73

Hover over nodes to see experimental data. Click on nodes to go to that point in the data below.

Proposed Target     Active Target     PCR Testing     Amplifer Generation     Orientation     Plasmid Purification     Expression     Protein Purification     NMR Structure     Refining     Validation     PDB Deposition     Proposed Target   Active Target   PCR Testing   Amplifer Generation   Orientation   Plasmid Purification   Expression   Protein Purification   NMR Structure   Refining   Validation   PDB Deposition

Status View   Chrono View

Name:	50S ribosomal protein L29.		Start Position:	1		End Position:	66
GNF Target Alias:	DL41:MH1-TM1492-tma-66-1-66		Sequence Type:	COMPLETE		Residues:	66
DNA Length:	198		Molecular Weight:	7972.04		Isoelectric Point:	10.27
Epsilon 280:	1490		Gravy Index:	-0.79		Species:	T. maritima
Activated:	2		Alanine Absolute:	3		Alanine Relative:	0.0455
Arginine Absolute:	9		Arginine Relative:	0.1364		Asparagine Absolute:	3
Asparagine Relative:	0.0455		Aspartic Acid Absolute:	2		Aspartic Acid Relative:	0.0303
Cysteine Absolute:	0		Cysteine Relative:	0.0000		Glutamic Acid Absolute:	8
Glutamic Acid Relative:	0.1212		Glutamine Absolute:	3		Glutamine Relative:	0.0455
Glycine Absolute:	2		Glycine Relative:	0.0303		Histidine Absolute:	0
Histidine Relative:	0.0000		Isoleucine Absolute:	5		Isoleucine Relative:	0.0758
Leucine Absolute:	12		Leucine Relative:	0.1818		Lysine Absolute:	8
Lysine Relative:	0.1212		Methionine Absolute:	3		Methionine Relative:	0.0455
Phenylalanine Absolute:	1		Phenylalanine Relative:	0.0152		Proline Absolute:	0
Proline Relative:	0.0000		Serine Absolute:	2		Serine Relative:	0.0303
Threonine Absolute:	4		Threonine Relative:	0.0606		Tryptophan Absolute:	0
Tryptophan Relative:	0.0000		Tyrosine Absolute:	1		Tyrosine Relative:	0.0152
Valine Absolute:	0		Valine Relative:	0.0000		Project:	GNF
Selection Date:	2001-08-10

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STAGE: Proposed Target

• Plate: 0 Cell: 283349 Date: 2001-08-10 Status: Success

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STAGE: Active Target

• Plate: 637 Cell: E10 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 0 Cell: 283349  
• Primer Plate: 637 Date: 2001-11-06
• Primer Well: E10 Date: 2001-11-06
• Sample Name: DL41:MH1-TM1492-tma-66-1-66 Date: 2006-09-16
• GNF Project Name: Thermotoga maritima Date: 2006-09-16
• GNF Program Name: JCSG Date: 2006-09-16
• Plate: 642 Cell: F5 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 0 Cell: 283349  
• Primer Plate: 642 Date: 2001-12-12
• Primer Well: F5 Date: 2001-12-12
• Sample Name: DL41:MH1-TM1492-tma-66-1-66 Date: 2006-09-16
• GNF Project Name: Thermotoga maritima Date: 2006-09-16
• GNF Program Name: JCSG Date: 2006-09-16

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STAGE: PCR Testing

• Plate: 637 Cell: E10 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 637 Cell: E10  
• Plate: 642 Cell: F5 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 642 Cell: F5  

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STAGE: Amplifer Generation

• Plate: 637 Cell: E10 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 637 Cell: E10  
• Plate: 642 Cell: F5 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 642 Cell: F5  

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STAGE: Orientation

• Plate: 637 Cell: E10 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 637 Cell: E10  
• Plate: 642 Cell: F5 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 642 Cell: F5  

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STAGE: Plasmid Purification

• Plate: 637 (C-0139) Cell: E10 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 637 (C-0139) Cell: E10  
• Clone ID: 7794 Date: 2002-06-25
• Strain/Vector: DL41 Date: 2006-09-16
• Clone Site: MH1 Date: 2006-09-16
• Cloned Date: 2001-08-10 Date: 2006-09-16
• Plate: 642 (C-0144) Cell: F5 Date: 2001-08-10 Status: Success - Backfilled      Previous Location: Plate: 642 (C-0144) Cell: F5  
• Clone ID: 8271 Date: 2002-06-25
• Strain/Vector: DL41 Date: 2006-09-16
• Clone Site: MH1 Date: 2006-09-16
• Cloned Date: 2001-08-10 Date: 2006-09-16

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STAGE: Expression

• Collection: 687 (E-0071) Cell: E10 Date: 2001-10-01 Status: Success - Backfilled      Previous Location: Plate: 637 (C-0139) Cell: E10  
• Fermentation Date: 2001-10-01 Date: 2006-09-12
• Protein ID: 5755 Date: 2002-06-25
• Media: Native-TB Date: 2006-09-12
• Plate: 694 (E-0082) Cell: F5 Date: 2001-10-08 Status: Success - Backfilled      Previous Location: Plate: 642 (C-0144) Cell: F5  
• Fermentation Date: 2001-10-08 Date: 2006-09-12
• Protein ID: 6746 Date: 2002-06-25
• Media: Native-TB Date: 2006-09-12
• Plate: 8454 (E-0145) Cell: F5 Date: 2002-08-21 Status: Success - Backfilled      Previous Location: Plate: 642 (C-0144) Cell: F5  
• Fermentation Date: 2002-08-21 Date: 2006-09-12
• Protein ID: 11707 Date: 2002-09-06
• Media: Native-TB Date: 2006-09-12
• Plate: 8454 (E-0145) Cell: F6 Date: 2002-08-21 Status: Success - Backfilled      Previous Location: Plate: 637 (C-0139) Cell: E10  
• Fermentation Date: 2002-08-21 Date: 2006-09-12
• Protein ID: 11708 Date: 2002-09-06
• Media: Native-TB Date: 2006-09-12

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STAGE: Protein Purification

• Collection: 687 (E-0071) Cell: E10 Date: 2001-11-13 Status: Success - Backfilled      Previous Location: Collection: 687 (E-0071) Cell: E10  
• Protein Version: 1 Date: 2001-11-13
• Purification Date: 2001-11-13 Date: 2003-08-11
• Plate: 694 (E-0082) Cell: F5 Date: 2001-12-19 Status: Success - Backfilled      Previous Location: Plate: 694 (E-0082) Cell: F5  
• Protein Version: 2 Date: 2001-12-19
• Purification Date: 2001-12-19 Date: 2003-08-11
• Plate: 8454 (E-0145) Cell: F5 Date: 2002-08-21 Status: Success - Backfilled      Previous Location: Plate: 8454 (E-0145) Cell: F5  
• Protein Version: 3 Date: 2002-08-21
• Purification Date: 2002-08-21 Date: 2003-08-11
• Plate: 8454 (E-0145) Cell: F6 Date: 2002-08-21 Status: Success - Backfilled      Previous Location: Plate: 8454 (E-0145) Cell: F6  
• Protein Version: 4 Date: 2002-08-21
• Purification Date: 2002-08-21 Date: 2003-08-11

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STAGE: NMR Structure

• Plate: 19957 Cell: 999 Date: 2003-10-17 Status: Success - Backfilled      Previous Location: Collection: 687 (E-0071) Cell: E10  
• Solver: Wolfgang Peti, Torsten Herrmann Date: 2003-03-15

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STAGE: Refining

• Collection: 19958 Cell: 999 Date: 2003-10-17 Status: Success - Backfilled      Previous Location: Plate: 19957 Cell: 999  

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STAGE: Validation

• Collection: 19959 Cell: 999 Date: 2003-10-17 Status: Success - Backfilled      Previous Location: Collection: 19958 Cell: 999  

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STAGE: PDB Deposition

• Collection: 29308 Cell: 999 Date: 2003-10-17 Status: Success - Backfilled      Previous Location: Collection: 19959 Cell: 999  
• PDB ID: 1r73 Date: 2005-02-15
• RCSB ID: PDB1R73 Date: 2005-02-15
• PDB File: C--Documents-and-Settings-bwest-Desktop-TM1492-1R73_pdb.pdb Date: 2005-02-15
• Coordinate mmCIF File: C--Documents-and-Settings-bwest-Desktop-TM1492-1R73_cif.cif Date: 2005-02-15
• Deposition Date: 2003-10-17 Date: 2005-02-15

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