JCSG Target History

		Search JCSG

Target ID:

393243

Target Name:

GS13738B

Protein Accession:

YP_211325.1

Local Accession:

GS13738B

cDNA Accession:

REF|NC_003228.3|:1965253-1966329

PSCA

PDB:3g3l

Hover over nodes to see experimental data. Click on nodes to go to that point in the data below.

Proposed Target

Active Target

PCR Testing

Amplifer Generation

Orientation

Plasmid Purification

Expression

Protein Purification

Crystallization

Crystal Hit

SDC Receiving

Screening

Data Collection

Integration

Scaling

Truncating

Phasing

Density Modification

Tracing

Refining

Validation

PDB Deposition

Proposed Target

Active Target

PCR Testing

Amplifer Generation

Orientation

Plasmid Purification

Expression

Protein Purification

Crystallization

Crystal Hit

SDC Receiving

Screening

Data Collection

Integration

Scaling

Truncating

Phasing

Density Modification

Tracing

Refining

Validation

PDB Deposition

Status View


Chrono View

Name:	Putative membrane-associated protein of unknown function.	Start Position:	33	End Position:	358
GNF Target Alias:	HK100:SpeedET-GS13738B-bfa-358-33-358	Sequence Type:	SEGMENT	Residues:	326
DNA Length:	978	Molecular Weight:	34517.78	Isoelectric Point:	4.71
Epsilon 280:	26820	Gravy Index:	-0.19	Species:	Bacteroides fragilis NCTC 9343
Activated:	0	Alanine Absolute:	38	Alanine Relative:	0.1166
Arginine Absolute:	3	Arginine Relative:	0.0092	Asparagine Absolute:	15
Asparagine Relative:	0.0460	Aspartic Acid Absolute:	19	Aspartic Acid Relative:	0.0583
Cysteine Absolute:	1	Cysteine Relative:	0.0031	Glutamic Acid Absolute:	21
Glutamic Acid Relative:	0.0644	Glutamine Absolute:	11	Glutamine Relative:	0.0337
Glycine Absolute:	26	Glycine Relative:	0.0798	Histidine Absolute:	4
Histidine Relative:	0.0123	Isoleucine Absolute:	15	Isoleucine Relative:	0.0460
Leucine Absolute:	18	Leucine Relative:	0.0552	Lysine Absolute:	22
Lysine Relative:	0.0675	Methionine Absolute:	6	Methionine Relative:	0.0184
Phenylalanine Absolute:	7	Phenylalanine Relative:	0.0215	Proline Absolute:	17
Proline Relative:	0.0521	Serine Absolute:	22	Serine Relative:	0.0675
Threonine Absolute:	29	Threonine Relative:	0.0890	Tryptophan Absolute:	0
Tryptophan Relative:	0.0000	Tyrosine Absolute:	18	Tyrosine Relative:	0.0552
Valine Absolute:	34	Valine Relative:	0.1043	Project:	GNF Autogenerated
Selection Date:	2007-09-11	PFAM:	pdb file loaded for [Hinxton #92427]

STAGE: Proposed Target

Plate: 0 Cell: 393243 Date: 2007-09-11 Status: Success

STAGE: Active Target

Plate: 62109 Cell: B01 Date: 2008-05-15 Status: Success Previous Location: Plate: 0 Cell: 393243

Sample Name: HK100:SpeedET-GS13738B-bfa-358-33-358 Date: 2008-06-08
GNF Project Name: PSI2_GutSecreted(GS) Date: 2008-06-08
GNF Program Name: JCSG Date: 2008-06-08

STAGE: PCR Testing

Plate: 62109 Cell: B01 Date: 2008-05-15 Status: Success Previous Location: Plate: 62109 Cell: B01

STAGE: Amplifer Generation

Plate: 62109 Cell: B01 Date: 2008-05-15 Status: Success Previous Location: Plate: 62109 Cell: B01

STAGE: Orientation

Plate: 62109 Cell: B01 Date: 2008-05-15 Status: Success Previous Location: Plate: 62109 Cell: B01

STAGE: Plasmid Purification

Plate: 62109 (C-0612) Cell: B01 Date: 2008-05-15 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Clone ID: 45331 Date: 2008-05-15
Strain/Vector: HK100 Date: 2008-06-08
Clone Site: SpeedET Date: 2008-06-08

STAGE: Expression

Plate: 62204 (E-1450) Cell: B01 Date: 2008-05-17 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Fermentation Date: 2008-05-23 Date: 2008-10-04
Protein ID: 135169 Date: 2008-05-17
Media: Native-TB Date: 2008-06-15

Plate: 65322 (E-1537) Cell: B01 Date: 2008-07-25 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Fermentation Date: 2008-07-25 Date: 2008-10-04
Protein ID: 144915 Date: 2008-07-29
Media: Small-scale (Mod. TB) Date: 2008-08-24

Plate: 68291 (E-1602) Cell: A06 Date: 2008-09-25 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Fermentation Date: 2008-09-25 Date: 2008-11-02
Protein ID: 152154 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-11-02

Plate: 68291 (E-1602) Cell: B06 Date: 2008-09-25 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Fermentation Date: 2008-09-25 Date: 2008-11-02
Protein ID: 152166 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-11-02

Plate: 68291 (E-1602) Cell: C06 Date: 2008-09-25 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Fermentation Date: 2008-09-25 Date: 2008-11-02
Protein ID: 152178 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-11-02

Plate: 68291 (E-1602) Cell: D06 Date: 2008-09-25 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Fermentation Date: 2008-09-25 Date: 2008-11-02
Protein ID: 152190 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-11-02

Plate: 68291 (E-1602) Cell: E06 Date: 2008-09-25 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Fermentation Date: 2008-09-25 Date: 2008-11-02
Protein ID: 152202 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-11-02

Plate: 68291 (E-1602) Cell: F06 Date: 2008-09-25 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Fermentation Date: 2008-09-25 Date: 2008-11-02
Protein ID: 152214 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-11-02

Plate: 68291 (E-1602) Cell: G06 Date: 2008-09-25 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Fermentation Date: 2008-09-25 Date: 2008-11-02
Protein ID: 152226 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-11-02

Plate: 68291 (E-1602) Cell: H06 Date: 2008-09-25 Status: Success Previous Location: Plate: 62109 (C-0612) Cell: B01

Fermentation Date: 2008-09-25 Date: 2008-11-02
Protein ID: 152238 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-11-02

STAGE: Protein Purification

Plate: 62204 (E-1450) Cell: B01 Date: 2008-05-17 Status: Success Previous Location: Plate: 62204 (E-1450) Cell: B01
Plate: 65322 (E-1537) Cell: B01 Date: 2008-07-29 Status: Success Previous Location: Plate: 65322 (E-1537) Cell: B01
Plate: 68291 (E-1602) Cell: A06 Date: 2008-09-26 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: A06

Purified Concentration (mg/mL): 9.1 Date: 2008-11-02

Plate: 68291 (E-1602) Cell: B06 Date: 2008-09-26 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: B06
Plate: 68291 (E-1602) Cell: C06 Date: 2008-09-26 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: C06
Plate: 68291 (E-1602) Cell: D06 Date: 2008-09-26 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: D06
Plate: 68291 (E-1602) Cell: E06 Date: 2008-09-26 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: E06
Plate: 68291 (E-1602) Cell: F06 Date: 2008-09-26 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: F06
Plate: 68291 (E-1602) Cell: G06 Date: 2008-09-26 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: G06
Plate: 68291 (E-1602) Cell: H06 Date: 2008-09-26 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: H06

STAGE: Crystallization

Plate: 70200 (G0046423) Cell: XT Date: 2008-11-06 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: A06

Screen Type: Sparse Matrix Date: 2008-12-07
Setup Date: 2008-11-06 Date: 2008-12-07
Setup Temp: 4 Date: 2008-12-07
Crystal Conditions: GNF1 Date: 2008-12-07
Drop Volume (nl): 200.0 Date: 2008-12-07
Precipitate Volume (nl): 200 Date: 2008-12-07
Reservoir Volume (ul): 50.0 Date: 2008-12-07

Plate: 70201 (G0046443) Cell: XT Date: 2008-11-06 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: A06

Screen Type: Sparse Matrix Date: 2008-12-07
Setup Date: 2008-11-06 Date: 2008-12-07
Setup Temp: 4 Date: 2008-12-07
Crystal Conditions: GNF2 Date: 2008-12-07
Drop Volume (nl): 200.0 Date: 2008-12-07
Precipitate Volume (nl): 200 Date: 2008-12-07
Reservoir Volume (ul): 50.0 Date: 2008-12-07

Plate: 70202 (G0045537) Cell: XT Date: 2008-11-06 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: A06

Screen Type: Sparse Matrix Date: 2008-12-07
Setup Date: 2008-11-06 Date: 2008-12-07
Setup Temp: 4 Date: 2008-12-07
Crystal Conditions: GNF3 Date: 2008-12-07
Drop Volume (nl): 200.0 Date: 2008-12-07
Precipitate Volume (nl): 200 Date: 2008-12-07
Reservoir Volume (ul): 50.0 Date: 2008-12-07

Plate: 70203 (G0045600) Cell: XT Date: 2008-11-06 Status: Success Previous Location: Plate: 68291 (E-1602) Cell: A06

Screen Type: Sparse Matrix Date: 2008-12-07
Setup Date: 2008-11-06 Date: 2008-12-07
Setup Temp: 4 Date: 2008-12-07
Crystal Conditions: GNF4 Date: 2008-12-07
Drop Volume (nl): 200.0 Date: 2008-12-07
Precipitate Volume (nl): 200 Date: 2008-12-07
Reservoir Volume (ul): 50.0 Date: 2008-12-07

STAGE: Crystal Hit

Plate: 70201 (G0046443) Cell: C02 Date: 2008-11-06 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: XT

Setup Date: 2008-11-06 Date: 2009-09-13
Screen Date: 2008-12-04 Date: 2009-09-13
Screen Conditions: GNF2 C02: 2.0000M (NH4)2SO4, 2.0000% PEG-400, 0.1M HEPES pH 7.5 Date: 2009-09-13
Crystal Classification: CRYSTAL HIT Date: 2009-09-13

Plate: 70201 (G0046443) Cell: C06 Date: 2008-11-06 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: XT

Setup Date: 2008-11-06 Date: 2009-09-13
Screen Date: 2008-12-04 Date: 2009-09-13
Screen Conditions: GNF2 C06: 2.0000M (NH4)2SO4, 0.2000M Li2SO4, 0.1M TRIS pH 7.0 Date: 2009-09-13
Crystal Classification: CRYSTAL HIT Date: 2009-09-13

Plate: 70201 (G0046443) Cell: E09 Date: 2008-11-06 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: XT

Setup Date: 2008-11-06 Date: 2009-09-13
Screen Date: 2008-12-04 Date: 2009-09-13
Screen Conditions: GNF2 E09: 0.5000M (NH4)2SO4, 1.0000M Li2SO4, 0.1M Citrate pH 5.6 Date: 2009-02-25
Crystal Classification: PROBABLY HARVESTABLE Date: 2009-02-25

Plate: 70201 (G0046443) Cell: F09 Date: 2008-11-06 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: XT

Setup Date: 2008-11-06 Date: 2009-09-13
Screen Date: 2008-12-04 Date: 2009-09-13
Screen Conditions: GNF2 F09: 1.6000M (NH4)2SO4, 0.1M Citrate pH 5.0 Date: 2009-02-25
Crystal Classification: PROBABLY HARVESTABLE Date: 2009-02-25

Plate: 70202 (G0045537) Cell: F03 Date: 2008-11-06 Status: Success Previous Location: Plate: 70202 (G0045537) Cell: XT

Setup Date: 2008-11-06 Date: 2008-12-21
Screen Date: 2008-11-20 Date: 2008-12-21
Screen Conditions: GNF3 F03: 35.0000% MPD, 0.1M Na,K-Phosphate pH 6.3 Date: 2008-12-21
Crystal Classification: PROBABLY HARVESTABLE Date: 2008-12-21

Plate: 70203 (G0045600) Cell: B02 Date: 2008-11-06 Status: Success Previous Location: Plate: 70203 (G0045600) Cell: XT

Setup Date: 2008-11-06 Date: 2008-12-21
Screen Date: 2008-11-20 Date: 2008-12-21
Screen Conditions: GNF4 B02: 3.2000M (NH4)2SO4, 0.1M Bicine pH 9.0 Date: 2008-12-21
Crystal Classification: CRYSTAL HIT Date: 2008-12-21

Plate: 70203 (G0045600) Cell: D09 Date: 2008-11-06 Status: Success Previous Location: Plate: 70203 (G0045600) Cell: XT

Setup Date: 2008-11-06 Date: 2008-12-21
Screen Date: 2008-11-20 Date: 2008-12-21
Screen Conditions: GNF4 D09: 10.0000% Glycerol, 1.2600M Na3Citrate, 0.1M HEPES pH 7.5 Date: 2008-12-21
Crystal Classification: CRYSTAL HIT Date: 2008-12-21

STAGE: SDC Receiving

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B1 Date: 2008-11-24 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: F09

Shipment ID: 1898 Date: 2008-11-24

Cryo Conditions: 20.00% Glycerol Date: 2008-11-24
Crystal Comments: rectangular Date: 2008-11-24
Ship Date: 2008-11-24 Date: 2008-11-24
Crystal Id: 104878 Date: 2008-11-24
Pin Position: 2574 Date: 2008-11-24
Ship Position: SSRL 00092 Date: 2008-11-24
Mount Date: 2008-11-21 Date: 2008-11-24

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B2 Date: 2008-11-24 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: F09

Shipment ID: 1898 Date: 2008-11-24

Cryo Conditions: 20.00% Glycerol Date: 2008-11-24
Crystal Comments: rectangular Date: 2008-11-24
Ship Date: 2008-11-24 Date: 2008-11-24
Crystal Id: 104879 Date: 2008-11-24
Pin Position: 314 Date: 2008-11-24
Ship Position: SSRL 00092 Date: 2008-11-24
Mount Date: 2008-11-21 Date: 2008-11-24

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B3 Date: 2008-11-24 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: F09

Shipment ID: 1898 Date: 2008-11-24

Cryo Conditions: 20.00% Glycerol Date: 2008-11-24
Crystal Comments: rectangular Date: 2008-11-24
Ship Date: 2008-11-24 Date: 2008-11-24
Crystal Id: 104880 Date: 2008-11-24
Pin Position: 374 Date: 2008-11-24
Ship Position: SSRL 00092 Date: 2008-11-24
Mount Date: 2008-11-21 Date: 2008-11-24

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B4 Date: 2008-11-24 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: F09

Shipment ID: 1898 Date: 2008-11-24

Cryo Conditions: 20.00% Glycerol Date: 2008-11-24
Crystal Comments: rectangular Date: 2008-11-24
Ship Date: 2008-11-24 Date: 2008-11-24
Crystal Id: 104881 Date: 2008-11-24
Pin Position: 2718 Date: 2008-11-24
Ship Position: SSRL 00092 Date: 2008-11-24
Mount Date: 2008-11-21 Date: 2008-11-24

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B5 Date: 2008-11-24 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: F09

Shipment ID: 1898 Date: 2008-11-24

Cryo Conditions: 20.00% Glycerol Date: 2008-11-24
Crystal Comments: rectangular Date: 2008-11-24
Ship Date: 2008-11-24 Date: 2008-11-24
Crystal Id: 104882 Date: 2008-11-24
Pin Position: 460 Date: 2008-11-24
Ship Position: SSRL 00092 Date: 2008-11-24
Mount Date: 2008-11-21 Date: 2008-11-24

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B6 Date: 2008-11-24 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: F09

Shipment ID: 1898 Date: 2008-11-24

Cryo Conditions: 20.00% Glycerol Date: 2008-11-24
Crystal Comments: rectangular Date: 2008-11-24
Ship Date: 2008-11-24 Date: 2008-11-24
Crystal Id: 104883 Date: 2008-11-24
Pin Position: 474 Date: 2008-11-24
Ship Position: SSRL 00092 Date: 2008-11-24
Stop Flag: 0 Date: 2009-01-29
SDC Stage: 2900 Date: 2009-02-02
Mount Date: 2008-11-21 Date: 2008-11-24

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B7 Date: 2008-11-24 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: F09

Shipment ID: 1898 Date: 2008-11-24

Cryo Conditions: 20.00% Glycerol Date: 2008-11-24
Crystal Comments: rectangular Date: 2008-11-24
Ship Date: 2008-11-24 Date: 2008-11-24
Crystal Id: 104884 Date: 2008-11-24
Pin Position: 590 Date: 2008-11-24
Ship Position: SSRL 00092 Date: 2008-11-24
Mount Date: 2008-11-21 Date: 2008-11-24

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B8 Date: 2008-11-24 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: F09

Shipment ID: 1898 Date: 2008-11-24

Cryo Conditions: 20.00% Glycerol Date: 2008-11-24
Crystal Comments: rectangular Date: 2008-11-24
Ship Date: 2008-11-24 Date: 2008-11-24
Crystal Id: 104885 Date: 2008-11-24
Pin Position: 454 Date: 2008-11-24
Ship Position: SSRL 00092 Date: 2008-11-24
Mount Date: 2008-11-21 Date: 2008-11-24

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: C1 Date: 2008-11-24 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: F09

Shipment ID: 1898 Date: 2008-11-24

Cryo Conditions: 20.00% Glycerol Date: 2008-11-24
Crystal Comments: rectangular Date: 2008-11-24
Ship Date: 2008-11-24 Date: 2008-11-24
Crystal Id: 104886 Date: 2008-11-24
Pin Position: 6896 Date: 2008-11-24
Ship Position: SSRL 00092 Date: 2008-11-24
Mount Date: 2008-11-21 Date: 2008-11-24

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: C2 Date: 2008-11-24 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: F09

Shipment ID: 1898 Date: 2008-11-24

Cryo Conditions: 20.00% Glycerol Date: 2008-11-24
Crystal Comments: rectangular Date: 2008-11-24
Ship Date: 2008-11-24 Date: 2008-11-24
Crystal Id: 104887 Date: 2008-11-24
Pin Position: 889 Date: 2008-11-24
Ship Position: SSRL 00092 Date: 2008-11-24
Mount Date: 2008-11-21 Date: 2008-11-24

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: E5 Date: 2008-12-15 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: E09

Shipment ID: 1914 Date: 2008-12-15

Cryo Conditions: 20.00% Glycerol Date: 2008-12-15
Crystal Comments: rod Date: 2008-12-15
Ship Date: 2008-12-15 Date: 2008-12-15
Crystal Id: 106800 Date: 2008-12-15
Pin Position: 1 Date: 2008-12-15
Ship Position: SSRL 00271 Date: 2008-12-15
Mount Date: 2008-12-12 Date: 2008-12-15

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: E6 Date: 2008-12-15 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: E09

Shipment ID: 1914 Date: 2008-12-15

Cryo Conditions: 20.00% Glycerol Date: 2008-12-15
Crystal Comments: rod Date: 2008-12-15
Ship Date: 2008-12-15 Date: 2008-12-15
Crystal Id: 106801 Date: 2008-12-15
Pin Position: 7047 Date: 2008-12-15
Ship Position: SSRL 00271 Date: 2008-12-15
Mount Date: 2008-12-12 Date: 2008-12-15

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: E7 Date: 2008-12-15 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: E09

Shipment ID: 1914 Date: 2008-12-15

Cryo Conditions: 20.00% Glycerol Date: 2008-12-15
Crystal Comments: rod Date: 2008-12-15
Ship Date: 2008-12-15 Date: 2008-12-15
Crystal Id: 106802 Date: 2008-12-15
Pin Position: 183 Date: 2008-12-15
Ship Position: SSRL 00271 Date: 2008-12-15
Mount Date: 2008-12-12 Date: 2008-12-15

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: E8 Date: 2008-12-15 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: E09

Shipment ID: 1914 Date: 2008-12-15

Cryo Conditions: 20.00% Glycerol Date: 2008-12-15
Crystal Comments: rod Date: 2008-12-15
Ship Date: 2008-12-15 Date: 2008-12-15
Crystal Id: 106803 Date: 2008-12-15
Pin Position: 4517 Date: 2008-12-15
Ship Position: SSRL 00271 Date: 2008-12-15
Mount Date: 2008-12-12 Date: 2008-12-15

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: F1 Date: 2008-12-15 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: E09

Shipment ID: 1914 Date: 2008-12-15

Cryo Conditions: 20.00% Glycerol Date: 2008-12-15
Crystal Comments: rod Date: 2008-12-15
Ship Date: 2008-12-15 Date: 2008-12-15
Crystal Id: 106804 Date: 2008-12-15
Pin Position: 2147 Date: 2008-12-15
Ship Position: SSRL 00271 Date: 2008-12-15
Mount Date: 2008-12-12 Date: 2008-12-15

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: F2 Date: 2008-12-15 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: E09

Shipment ID: 1914 Date: 2008-12-15

Cryo Conditions: 20.00% Glycerol Date: 2008-12-15
Crystal Comments: rod Date: 2008-12-15
Ship Date: 2008-12-15 Date: 2008-12-15
Crystal Id: 106805 Date: 2008-12-15
Pin Position: 2105 Date: 2008-12-15
Ship Position: SSRL 00271 Date: 2008-12-15
Mount Date: 2008-12-12 Date: 2008-12-15

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: F3 Date: 2008-12-15 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: E09

Shipment ID: 1914 Date: 2008-12-15

Cryo Conditions: 20.00% Glycerol Date: 2008-12-15
Crystal Comments: rod Date: 2008-12-15
Ship Date: 2008-12-15 Date: 2008-12-15
Crystal Id: 106806 Date: 2008-12-15
Pin Position: 2079 Date: 2008-12-15
Ship Position: SSRL 00271 Date: 2008-12-15
Mount Date: 2008-12-12 Date: 2008-12-15

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: F4 Date: 2008-12-15 Status: Success Previous Location: Plate: 70201 (G0046443) Cell: E09

Shipment ID: 1914 Date: 2008-12-15

Cryo Conditions: 20.00% Glycerol Date: 2008-12-15
Crystal Comments: rod Date: 2008-12-15
Ship Date: 2008-12-15 Date: 2008-12-15
Crystal Id: 106807 Date: 2008-12-15
Pin Position: 4067 Date: 2008-12-15
Ship Position: SSRL 00271 Date: 2008-12-15
Mount Date: 2008-12-12 Date: 2008-12-15

STAGE: Screening

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B1 Date: 2008-11-30 Status: Success

Image Directory: /data/jcsg/ssrl4/11_1/20081130/screening/cassette92/GS13738B Date: 2008-12-19
Xtal Quality: 7 Date: 2008-12-19
Ice Rings: 0 Date: 2008-12-19
Diffraction Quality: 1 Date: 2008-12-19
Single Xtal: 1 Date: 2008-12-19
Spot Shape: 9 Date: 2008-12-19
Maximum Resolution: 2.8 Date: 2008-12-19
Unit Cell Length: 107,107,89 Date: 2008-12-19
Unit Cell Angles: 90,90,120 Date: 2008-12-19
Beamline: SSRL BL11-1 Date: 2008-12-19
Space Group: P3 Date: 2008-12-19
Robot Y or N: 1 Date: 2008-12-19
Screen Date: 2008-11-30 Date: 2008-12-19
Freezing: CLEAR Date: 2008-12-19
Loop: OK Date: 2008-12-19
Crystal Condition: SINGLE Date: 2008-12-19

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B2 Date: 2008-11-30 Status: Success

Image Directory: /data/jcsg/ssrl4/11_1/20081130/screening/cassette92/GS13738B Date: 2008-12-19
Xtal Quality: 7 Date: 2008-12-19
Ice Rings: 0 Date: 2008-12-19
Diffraction Quality: 3 Date: 2008-12-19
Single Xtal: ? Date: 2008-12-19
Spot Shape: 9 Date: 2008-12-19
Maximum Resolution: 2.5 Date: 2008-12-19
Unit Cell Length: 107,107,89 Date: 2008-12-19
Unit Cell Angles: 90,90,120 Date: 2008-12-19
Mosaicity: 0.5 Date: 2008-12-19
Beamline: SSRL BL11-1 Date: 2008-12-19
Space Group: P3 Date: 2008-12-19
Robot Y or N: 1 Date: 2008-12-19
Screen Date: 2008-11-30 Date: 2008-12-19
Freezing: CLEAR Date: 2008-12-19
Loop: OK Date: 2008-12-19
Crystal Condition: CND Date: 2008-12-19

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B3 Date: 2008-11-30 Status: Success

Image Directory: /data/jcsg/ssrl4/11_1/20081130/screening/cassette92/GS13738B Date: 2008-12-19
Xtal Quality: 7 Date: 2008-12-19
Ice Rings: 0 Date: 2008-12-19
Diffraction Quality: 1 Date: 2008-12-19
Single Xtal: 1 Date: 2008-12-19
Spot Shape: 9 Date: 2008-12-19
Maximum Resolution: 2.6 Date: 2008-12-19
Unit Cell Length: 107,107,89 Date: 2008-12-19
Unit Cell Angles: 90,90,120 Date: 2008-12-19
Mosaicity: 0.55 Date: 2008-12-19
Beamline: SSRL BL11-1 Date: 2008-12-19
Space Group: P3 Date: 2008-12-19
Robot Y or N: 1 Date: 2008-12-19
Screen Date: 2008-11-30 Date: 2008-12-19
Freezing: CLEAR Date: 2008-12-19
Loop: OK Date: 2008-12-19
Crystal Condition: CND Date: 2008-12-19

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B4 Date: 2008-11-30 Status: Success

Image Directory: /data/jcsg/ssrl4/11_1/20081130/screening/cassette92/GS13738B Date: 2008-12-01
Xtal Quality: 6 Date: 2008-12-01
Ice Rings: 0 Date: 2008-12-01
Diffraction Quality: 1 Date: 2008-12-01
Single Xtal: 1 Date: 2008-12-01
Spot Shape: 8 Date: 2008-12-01
Maximum Resolution: 3 Date: 2008-12-01
Beamline: SSRL BL11-1 Date: 2008-12-01
Robot Y or N: 1 Date: 2008-12-01
Screen Date: 2008-11-30 Date: 2008-12-01
Freezing: CLEAR Date: 2008-12-01
Loop: OK Date: 2008-12-01
Crystal Condition: SINGLE Date: 2008-12-01

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B5 Date: 2008-11-30 Status: Success

Image Directory: /data/jcsg/ssrl4/11_1/20081130/screening/cassette92/GS13738B Date: 2008-12-19
Xtal Quality: 7 Date: 2008-12-19
Ice Rings: 0 Date: 2008-12-19
Diffraction Quality: 1 Date: 2008-12-19
Single Xtal: ? Date: 2008-12-19
Spot Shape: 9 Date: 2008-12-19
Maximum Resolution: 2.7 Date: 2008-12-19
Unit Cell Length: 107,107,89 Date: 2008-12-19
Unit Cell Angles: 90,90,120 Date: 2008-12-19
Beamline: SSRL BL11-1 Date: 2008-12-19
Space Group: P3 Date: 2008-12-19
Robot Y or N: 1 Date: 2008-12-19
Screen Date: 2008-11-30 Date: 2008-12-19
Freezing: CLEAR Date: 2008-12-19
Loop: OK Date: 2008-12-19
Crystal Condition: CND Date: 2008-12-19

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B6 Date: 2008-11-30 Status: Success

Image Directory: /data/jcsg/ssrl4/11_1/20081130/screening/cassette92/GS13738B Date: 2010-01-26
Xtal Quality: 8 Date: 2010-01-26
Ice Rings: 0 Date: 2010-01-26
Diffraction Quality: 2 Date: 2010-01-26
Single Xtal: 1 Date: 2010-01-26
Spot Shape: 9 Date: 2010-01-26
Maximum Resolution: 2.4 Date: 2010-01-26
Unit Cell Length: 107,107,89 Date: 2010-01-26
Unit Cell Angles: 90,90,120 Date: 2010-01-26
Mosaicity: 0.4 Date: 2010-01-26
Beamline: SSRL BL11-1 Date: 2010-01-26
Space Group: P3 Date: 2010-01-26
Robot Y or N: 1 Date: 2010-01-26
Screen Date: 2008-11-30 Date: 2010-01-26
Freezing: CLEAR Date: 2010-01-26
Loop: OK Date: 2010-01-26
Crystal Condition: CND Date: 2010-01-26
Comments: crystal lost on 2009/07/20 Date: 2010-01-26

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B7 Date: 2008-11-30 Status: Success

Image Directory: /data/jcsg/ssrl4/11_1/20081130/screening/cassette92/GS13738B Date: 2008-12-01
Xtal Quality: 2 Date: 2008-12-01
Ice Rings: 0 Date: 2008-12-01
Diffraction Quality: 1 Date: 2008-12-01
Single Xtal: 1 Date: 2008-12-01
Spot Shape: 8 Date: 2008-12-01
Maximum Resolution: 6.7 Date: 2008-12-01
Beamline: SSRL BL11-1 Date: 2008-12-01
Robot Y or N: 1 Date: 2008-12-01
Screen Date: 2008-11-30 Date: 2008-12-01
Freezing: CLEAR Date: 2008-12-01
Loop: OK Date: 2008-12-01
Crystal Condition: CND Date: 2008-12-01

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B8 Date: 2008-11-30 Status: No Diffraction

Image Directory: /data/jcsg/ssrl4/11_1/20081130/screening/cassette92/GS13738B Date: 2008-12-01
Xtal Quality: 0 Date: 2008-12-01
Ice Rings: 5 Date: 2008-12-01
Diffraction Quality: 0 Date: 2008-12-01
Beamline: SSRL BL11-1 Date: 2008-12-01
Robot Y or N: 1 Date: 2008-12-01
Screen Date: 2008-11-30 Date: 2008-12-01
Freezing: FROST Date: 2008-12-01
Loop: OK Date: 2008-12-01
Crystal Condition: SINGLE Date: 2008-12-01
Comments: surface ice Date: 2008-12-01

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: C1 Date: 2008-11-30 Status: Success

Image Directory: /data/jcsg/ssrl4/11_1/20081130/screening/cassette92/GS13738B Date: 2008-12-01
Xtal Quality: 4 Date: 2008-12-01
Ice Rings: 8 Date: 2008-12-01
Diffraction Quality: 1 Date: 2008-12-01
Single Xtal: 0 Date: 2008-12-01
Spot Shape: 7 Date: 2008-12-01
Maximum Resolution: 2.6 Date: 2008-12-01
Beamline: SSRL BL11-1 Date: 2008-12-01
Robot Y or N: 1 Date: 2008-12-01
Screen Date: 2008-11-30 Date: 2008-12-01
Freezing: FROST Date: 2008-12-01
Loop: OK Date: 2008-12-01
Crystal Condition: CND Date: 2008-12-01
Comments: surface ice Date: 2008-12-01

Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: C2 Date: 2008-11-30 Status: Success

Image Directory: /data/jcsg/ssrl4/11_1/20081130/screening/cassette92/GS13738B Date: 2008-12-01
Xtal Quality: 7 Date: 2008-12-01
Ice Rings: 8 Date: 2008-12-01
Diffraction Quality: 1 Date: 2008-12-01
Single Xtal: 1 Date: 2008-12-01
Spot Shape: 8 Date: 2008-12-01
Maximum Resolution: 2.8 Date: 2008-12-01
Beamline: SSRL BL11-1 Date: 2008-12-01
Robot Y or N: 1 Date: 2008-12-01
Screen Date: 2008-11-30 Date: 2008-12-01
Freezing: CLEAR Date: 2008-12-01
Loop: OK Date: 2008-12-01
Crystal Condition: SINGLE Date: 2008-12-01

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: E5 Date: 2009-01-08 Status: Success

Image Directory: /data/jcsg/ssrl/9_1/20090105/screening/cassette271/GS13738B Date: 2009-01-13
Xtal Quality: 4 Date: 2009-01-13
Ice Rings: 5 Date: 2009-01-13
Diffraction Quality: 1 Date: 2009-01-13
Single Xtal: 1 Date: 2009-01-13
Spot Shape: 8 Date: 2009-01-13
Maximum Resolution: 4.3 Date: 2009-01-13
Beamline: SSRL BL9-1 Date: 2009-01-13
Robot Y or N: 1 Date: 2009-01-13
Screen Date: 2009-01-08 Date: 2009-01-13
Freezing: CLEAR Date: 2009-01-13
Loop: OK Date: 2009-01-13
Crystal Condition: CND Date: 2009-01-13

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: E6 Date: 2009-01-08 Status: Success

Image Directory: /data/jcsg/ssrl/9_1/20090105/screening/cassette271/GS13738B Date: 2010-01-26
Xtal Quality: 7 Date: 2010-01-26
Ice Rings: 0 Date: 2010-01-26
Diffraction Quality: 1 Date: 2010-01-26
Single Xtal: 1 Date: 2010-01-26
Spot Shape: 8 Date: 2010-01-26
Maximum Resolution: 2.7 Date: 2010-01-26
Unit Cell Length: 108,108,89 Date: 2010-01-26
Unit Cell Angles: 90,90,120 Date: 2010-01-26
Mosaicity: 0.8 Date: 2010-01-26
Beamline: SSRL BL9-1 Date: 2010-01-26
Space Group: P3 Date: 2010-01-26
Robot Y or N: 1 Date: 2010-01-26
Screen Date: 2009-01-08 Date: 2010-01-26
Freezing: CLEAR Date: 2010-01-26
Loop: OK Date: 2010-01-26
Crystal Condition: SINGLE Date: 2010-01-26
Comments: crystal lost on 2009/07/20 Date: 2010-01-26

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: E7 Date: 2009-01-08 Status: Success

Image Directory: /data/jcsg/ssrl/9_1/20090105/screening/cassette271/GS13738B Date: 2010-01-26
Xtal Quality: 7 Date: 2010-01-26
Ice Rings: 2 Date: 2010-01-26
Diffraction Quality: 1 Date: 2010-01-26
Single Xtal: 1 Date: 2010-01-26
Spot Shape: 9 Date: 2010-01-26
Maximum Resolution: 2.7 Date: 2010-01-26
Unit Cell Length: 108,108,89 Date: 2010-01-26
Unit Cell Angles: 90,90,120 Date: 2010-01-26
Mosaicity: 0.3 Date: 2010-01-26
Beamline: SSRL BL9-1 Date: 2010-01-26
Space Group: P3 Date: 2010-01-26
Robot Y or N: 1 Date: 2010-01-26
Screen Date: 2009-01-08 Date: 2010-01-26
Freezing: FROST Date: 2010-01-26
Loop: OK Date: 2010-01-26
Crystal Condition: CND Date: 2010-01-26
Comments: surface ice,crystal lost on 2009/07/20 Date: 2010-01-26

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: E8 Date: 2009-01-08 Status: Success

Image Directory: /data/jcsg/ssrl/9_1/20090105/screening/cassette271/GS13738B Date: 2009-01-13
Xtal Quality: 6 Date: 2009-01-13
Ice Rings: 0 Date: 2009-01-13
Diffraction Quality: 1 Date: 2009-01-13
Single Xtal: ? Date: 2009-01-13
Spot Shape: 8 Date: 2009-01-13
Maximum Resolution: 3 Date: 2009-01-13
Beamline: SSRL BL9-1 Date: 2009-01-13
Robot Y or N: 1 Date: 2009-01-13
Screen Date: 2009-01-08 Date: 2009-01-13
Freezing: CLEAR Date: 2009-01-13
Loop: OK Date: 2009-01-13
Crystal Condition: CND Date: 2009-01-13

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: F1 Date: 2009-01-08 Status: No Diffraction

Image Directory: /data/jcsg/ssrl/9_1/20090105/screening/cassette271/GS13738B Date: 2009-01-13
Xtal Quality: 0 Date: 2009-01-13
Ice Rings: 0 Date: 2009-01-13
Diffraction Quality: 0 Date: 2009-01-13
Beamline: SSRL BL9-1 Date: 2009-01-13
Robot Y or N: 1 Date: 2009-01-13
Screen Date: 2009-01-08 Date: 2009-01-13
Freezing: CLEAR Date: 2009-01-13
Loop: OK Date: 2009-01-13
Crystal Condition: CND Date: 2009-01-13

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: F2 Date: 2009-01-08 Status: No Diffraction

Image Directory: /data/jcsg/ssrl/9_1/20090105/screening/cassette271/GS13738B Date: 2009-01-13
Xtal Quality: 0 Date: 2009-01-13
Ice Rings: 3 Date: 2009-01-13
Diffraction Quality: 0 Date: 2009-01-13
Beamline: SSRL BL9-1 Date: 2009-01-13
Robot Y or N: 1 Date: 2009-01-13
Screen Date: 2009-01-08 Date: 2009-01-13
Freezing: FROST Date: 2009-01-13
Loop: OK Date: 2009-01-13
Crystal Condition: CND Date: 2009-01-13
Comments: surface ice Date: 2009-01-13

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: F3 Date: 2009-01-08 Status: No Diffraction

Image Directory: /data/jcsg/ssrl/9_1/20090105/screening/cassette271/GS13738B Date: 2009-01-13
Xtal Quality: 0 Date: 2009-01-13
Ice Rings: 0 Date: 2009-01-13
Diffraction Quality: 0 Date: 2009-01-13
Beamline: SSRL BL9-1 Date: 2009-01-13
Robot Y or N: 1 Date: 2009-01-13
Screen Date: 2009-01-08 Date: 2009-01-13
Freezing: CLEAR Date: 2009-01-13
Loop: OK Date: 2009-01-13
Crystal Condition: CND Date: 2009-01-13

Dewar: 71543 (Fox-12/15/2008) Cassette: SSRL 00271 (SSRL 00271Fox-12/15/2008) Cell: F4 Date: 2009-01-08 Status: Success

Image Directory: /data/jcsg/ssrl/9_1/20090105/screening/cassette271/GS13738B Date: 2010-01-26
Xtal Quality: 7 Date: 2010-01-26
Ice Rings: 0 Date: 2010-01-26
Diffraction Quality: 1 Date: 2010-01-26
Single Xtal: 1 Date: 2010-01-26
Spot Shape: 9 Date: 2010-01-26
Maximum Resolution: 2.6 Date: 2010-01-26
Unit Cell Length: 108,108,89 Date: 2010-01-26
Unit Cell Angles: 90,90,120 Date: 2010-01-26
Mosaicity: 0.3 Date: 2010-01-26
Beamline: SSRL BL9-1 Date: 2010-01-26
Space Group: P3 Date: 2010-01-26
Robot Y or N: 1 Date: 2010-01-26
Screen Date: 2009-01-08 Date: 2010-01-26
Freezing: CLEAR Date: 2010-01-26
Loop: OK Date: 2010-01-26
Crystal Condition: CND Date: 2010-01-26
Comments: crystal lost on 2009/07/20 Date: 2010-01-26

STAGE: Data Collection

Collection: 71354 Cell: 1 Date: 2008-12-07 Status: Success Previous Location: Dewar: 70849 (Falcon-11/24/2008) Cassette: SSRL 00092 (SSRL 00092Falcon-11/24/2008) Cell: B6

CollectData.xml: CollectData.xml Date: 2008-12-10
Beamline.xml: beamline.xml Date: 2008-12-10
Notes file: notes.txt Date: 2008-12-10
Beamline: BL9-2 Date: 2008-12-10
ExperimentType: X-Ray Date: 2008-12-10
Program name: Blu-Ice Date: 2008-12-10
Program version: 4.0 Date: 2008-12-10
Synchrotron: SSRL Date: 2008-12-10
Detector name: MAR325 Date: 2008-12-10
Detector type: CCD Date: 2008-12-10
Detector serial: 11 Date: 2008-12-10
Detector manufacturer: MAR Date: 2008-12-10
BeamlineInfoURL: http://biosync.sdsc.edu/ssrl/BL9-2.html Date: 2008-12-10
MOSFLM_DETECTOR_PARAMETERS: !commented out due to use of new mosflm !DETECTOR ROTATION HORIZ ANTI FAST HORIZONTAL ORIGIN UR rectangular TYPE MARCCD !SIZE 4096 4096 HEADER 1 byte 4096 !LIMITS XMIN 0 XMAX 162.5 YMIN 0 YMAX 162.5 xscan 2048 yscan 2048 GAIN 0.3 Date: 2008-12-10
MOSFLM_BEAMLINE_PARAMETERS: SYNCHROTRON POLARIZATION 0.92 DIVERGENCE 0.250 0.030 DISPERSION 0.00020 Date: 2008-12-10
DC Date: 2008-12-07 Date: 2008-12-10
Performed By: Qingping Xu Date: 2008-12-10
Temperature (K): 100 Date: 2008-12-10
Beamline: BL9-2 Date: 2008-12-10
ImageDirectory: /data/jcsg/ssrl4/9_2/20081207/collection/GS13738B/104883/ Date: 2008-12-10
Description: l1s1 Date: 2008-12-10
Wavelength: 0.97966 Date: 2008-12-10
Anomalous fprimv: -8.3 Date: 2008-12-10
Anomalous fprprv: 6.1 Date: 2008-12-10
DetectorMode: undefined Date: 2008-12-10
Distance: 250.00 Date: 2008-12-10
BeamCenter X: 162.5 Date: 2008-12-10
BeamCenter Y: 162.5 Date: 2008-12-10
ImagePrefix: 104883_1 Date: 2008-12-10
ImageSuffix: mccd Date: 2008-12-10
PhiDelta: 0.50 Date: 2008-12-10
PhiStart: 183.00 Date: 2008-12-10
FrameNumber start: 1 Date: 2008-12-10
FrameNumber end: 240 Date: 2008-12-10

STAGE: Integration

Collection: 71355 Cell: 1 Date: 2008-12-10 Status: Success Previous Location: Collection: 71354 Cell: 1

Integration Log File: integrate.log Date: 2008-12-10
Integration Reflection File: integrate.mtz Date: 2008-12-10
Integration Name: l1i Date: 2008-12-10
Performed By: 79 Date: 2008-12-10
Integration Program: MOSFLM Date: 2008-12-10
Program Version: 7.0.1 Date: 2008-12-10
Integration Date: 2008-12-10 Date: 2008-12-10

STAGE: Scaling

Collection: 71356 Cell: 1 Date: 2008-12-10 Status: Success Previous Location: Collection: 71355 Cell: 1

Scale Log File: scale.log Date: 2008-12-10
Scale Reflection File: scale.mtz Date: 2008-12-10
Scale Name: l1s Date: 2008-12-10
Performed By: 79 Date: 2008-12-10
Scaling program: SCALA Date: 2008-12-10
Program Version: 3.2.5 Date: 2008-12-10
Space group: P321 Date: 2008-12-10
Cell A: 107.6214 Date: 2008-12-10
Cell B: 107.6214 Date: 2008-12-10
Cell C: 89.2955 Date: 2008-12-10
Angle Alpha: 90.0000 Date: 2008-12-10
Angle Beta: 90.0000 Date: 2008-12-10
Angle Gamma: 120.0000 Date: 2008-12-10
High resolution overall: 2.20 Date: 2008-12-10
Low resolution overall: 50.00 Date: 2008-12-10
Completeness overall: 100.0 Date: 2008-12-10
Redundancy overall: 7.4 Date: 2008-12-10
Rsym(I) overall: 0.134 Date: 2008-12-10
I/sigma(I) overall: 5.1 Date: 2008-12-10
High resolution limit for highest resolution shell: 2.20 Date: 2008-12-10
Low resolution limit for highest resolution shell: 2.26 Date: 2008-12-10
Completeness shell: 100.0 Date: 2008-12-10
Redundancy shell: 7.5 Date: 2008-12-10
Rsym(I) shell: 0.931 Date: 2008-12-10
I/sigma(I) shell: 0.8 Date: 2008-12-10
Observed reflections overall: 227775 Date: 2008-12-10
Unmerged HKL File: nomerge.sca Date: 2008-12-10
Scaling Date: 2008-12-10 Date: 2008-12-10
Number of reflections: 227775 Date: 2008-12-10

STAGE: Truncating

Collection: 71357 Cell: 1 Date: 2008-12-10 Status: Success Previous Location: Collection: 71356 Cell: 1

Truncate Name: l1t Date: 2008-12-10
Performed By: 79 Date: 2008-12-10
Truncate Log File: truncate.log Date: 2008-12-10
Truncate Reflection File: truncate.mtz Date: 2008-12-10
truncate program version: 5.0 Date: 2008-12-10
Unique reflections overall: 368724 Date: 2008-12-10
Wilson_Bfact: 37.005 Date: 2008-12-10
Truncate Date: 2008-12-10 Date: 2008-12-10
Cell A: 107.6214 Date: 2008-12-10
Cell B: 107.6214 Date: 2008-12-10
Cell C: 89.2955 Date: 2008-12-10
Angle Alpha: 90.0000 Date: 2008-12-10
Angle Beta: 90.0000 Date: 2008-12-10
Angle Gamma: 120.0000 Date: 2008-12-10
Unique Reflections: 30727 Date: 2008-12-10
Resolution Range Low: 29.761 Date: 2008-12-10
Resolution Range High: 2.200 Date: 2008-12-10

STAGE: Phasing

Collection: 71358 Cell: 1 Date: 2008-12-10 Status: Success Previous Location: Collection: 71357 Cell: 1

Name of phasing run: shelx-sharp Date: 2008-12-10
Performed By: 79 Date: 2008-12-10
Phasing reflection file: solve.mtz Date: 2008-12-10
Phasing PRT log: ha.pdb Date: 2008-12-10
Phasing log file: sharp_log.mmcif Date: 2008-12-10
Solution Type: SAD Date: 2008-12-10
Phasing Date: 2008-12-10 Date: 2008-12-10

STAGE: Density Modification

Collection: 71359 Cell: 1 Date: 2008-12-10 Status: Success Previous Location: Collection: 71357 Cell: 1 Collection: 71358 Cell: 1

Name of DM run: solomon Date: 2008-12-10
Performed By: 79 Date: 2008-12-10
DM reflection file: solomon.mtz Date: 2008-12-10
DM log file: solomon_log.html Date: 2008-12-10
DM Date: 2008-12-10 Date: 2008-12-10

STAGE: Tracing

Collection: 71360 Cell: 1 Date: 2008-12-10 Status: Solved Structure Previous Location: Collection: 71357 Cell: 1 Collection: 71359 Cell: 1 Collection: 71358 Cell: 1

Name of tracing run: warp Date: 2008-12-10
Performed By: 79 Date: 2008-12-10
Tracing log file: 2008000108_warp_log.html Date: 2008-12-10
Tracing reflection file: 2008000108_warpNtrace.mtz Date: 2008-12-10
Tracing PDB: 2008000108_warpNtrace.pdb Date: 2008-12-10
Tracing Date: 2008-12-10 Date: 2008-12-10

Collection: 71360 Cell: 2 Date: 2008-12-10 Status: Solved Structure Previous Location: Collection: 71360 Cell: 1 Collection: 71357 Cell: 1 Collection: 71359 Cell: 1 Collection: 71358 Cell: 1

Name of tracing run: cnsnss Date: 2008-12-10
Performed By: 79 Date: 2008-12-10
Tracing Comments: 295/358 traced/docked, complete Date: 2008-12-10
Tracing log file: consensus_p3221_nmol1_final_Xpleo_refmac.log Date: 2008-12-10
Tracing reflection file: consensus_p3221_nmol1_final_Xpleo_refmac.mtz Date: 2008-12-10
Tracing PDB: consensus_p3221_nmol1_final_Xpleo_refmac.pdb Date: 2008-12-10
Tracing Date: 2008-12-10 Date: 2008-12-10

STAGE: Refining

Collection: 71498 Cell: 1 Date: 2008-12-11 Status: Success Previous Location: Collection: 71360 Cell: 2 Collection: 71357 Cell: 1 Collection: 71359 Cell: 1 Collection: 71358 Cell: 1

Name of refinement run: ref1 Date: 2008-12-11
Performed By: 2793 Date: 2008-12-11
REFINEMENT TARGET :: MAXIMUM LIKELIHOOD WITH PHASES Date: 2008-12-11
RESOLUTION RANGE HIGH:: 2.20 Date: 2008-12-11
DATA CUTOFF (SIGMA(F)) :: NONE Date: 2008-12-11
COMPLETENESS (WORKING+TEST) (%) :: 99.94 Date: 2008-12-11
NUMBER OF REFLECTIONS :: 29154 Date: 2008-12-11
CROSS-VALIDATION METHOD :: THROUGHOUT Date: 2008-12-11
FREE R VALUE TEST SET SELECTION :: RANDOM Date: 2008-12-11
R VALUE (WORKING SET) :: 0.18022 Date: 2008-12-11
FREE R VALUE :: 0.22152 Date: 2008-12-11
FREE R VALUE TEST SET SIZE (%) :: 5.0 Date: 2008-12-11
FREE R VALUE TEST SET COUNT :: 1548 Date: 2008-12-11
TOTAL NUMBER OF BINS USED :: 20 Date: 2008-12-11
BIN RESOLUTION RANGE HIGH (A) :: 2.200 Date: 2008-12-11
BIN RESOLUTION RANGE LOW (A) :: 2.257 Date: 2008-12-11
REFLECTIONS IN BIN (WORKING SET) :: 2118 Date: 2008-12-11
BIN R VALUE (WORKING SET) :: 0.242 Date: 2008-12-11
BIN FREE R VALUE :: 0.274 Date: 2008-12-11
BIN FREE R VALUE TEST SET COUNT :: 116 Date: 2008-12-11
MEAN B VALUE (OVERALL, A**2) :: 41.946 Date: 2008-12-11
OVERALL ANISOTROPIC B VALUE B11 (A**2) :: 1.52 Date: 2008-12-11
OVERALL ANISOTROPIC B VALUE B22 (A**2) :: 1.52 Date: 2008-12-11
OVERALL ANISOTROPIC B VALUE B33 (A**2) :: -2.28 Date: 2008-12-11
OVERALL ANISOTROPIC B VALUE B12 (A**2) :: 0.76 Date: 2008-12-11
OVERALL ANISOTROPIC B VALUE B13 (A**2) :: 0.00 Date: 2008-12-11
OVERALL ANISOTROPIC B VALUE B23 (A**2) :: 0.00 Date: 2008-12-11
SPACE GROUP: P3221 Date: 2008-12-11
MAIN-CHAIN ANGLE RMS (A**2) :: 1.653 Date: 2008-12-11
BOND LENGTHS RMS DEVIATIONS (A) :: 0.017 Date: 2008-12-11
Refinement Log: submitted1.log Date: 2008-12-11
Reflection File: submitted1.mtz Date: 2008-12-11
PDB file: submitted1.pdb Date: 2008-12-11
Program: REFMAC Date: 2008-12-11
Version: 5.4.0067 Date: 2008-12-11
Cell A: 107.621 Date: 2008-12-11
Cell B: 107.621 Date: 2008-12-11
Cell C: 89.296 Date: 2008-12-11
Angle Alpha: 90.00 Date: 2008-12-11
Angle Beta: 90.00 Date: 2008-12-11
Angle Gamma: 120.00 Date: 2008-12-11
ALL ATOMS: 2350 Date: 2008-12-11
ESU BASED ON R VALUE: 0.139 Date: 2008-12-11
ESU BASED ON FREE R VALUE: 0.141 Date: 2008-12-11
ESU BASED ON MAXIMUM LIKELIHOOD: 0.101 Date: 2008-12-11
ESU FOR B VALUE BASED ON MAXIMUM LIKELIHOOD: 7.799 Date: 2008-12-11
CORRELATION COEFFICIENT FO-FC: 0.958 Date: 2008-12-11
CORRELATION COEFFICIENT FO-FC FREE: 0.936 Date: 2008-12-11
BOND LENGTHS REFINED ATOMS - LENGTH COUNT: 2197 Date: 2008-12-11
BOND LENGTHS REFINED ATOMS - LENGTH WEIGHT: 0.022 Date: 2008-12-11
BOND ANGLES REFINED ATOMS - ANGLE WEIGHT: 1.979 Date: 2008-12-11
CHIRAL-CENTER RESTRAINTS RMS: 0.089 Date: 2008-12-11
TORSION ANGLES, PERIOD COUNT: 288::79::357::1 Date: 2008-12-11
TORSION ANGLES, PERIOD RMS: 3.838::32.192::10.372::6.304 Date: 2008-12-11
TORSION ANGLES, PERIOD WEIGHT: 5.000::26.709::15.000::15.000 Date: 2008-12-11
CHIRAL-CENTER RESTRAINTS COUNT: 361 Date: 2008-12-11
CHIRAL-CENTER RESTRAINTS WEIGHT: 0.200 Date: 2008-12-11
GENERAL PLANES REFINED ATOMS COUNT: 2438 Date: 2008-12-11
GENERAL PLANES REFINED ATOMS RMS: 0.008 Date: 2008-12-11
GENERAL PLANES REFINED ATOMS WEIGHT: 0.021 Date: 2008-12-11
MAIN-CHAIN BOND REFINED ATOMS COUNT: 1443 Date: 2008-12-11
MAIN-CHAIN BOND REFINED ATOMS RMS: 1.243 Date: 2008-12-11
MAIN-CHAIN BOND REFINED ATOMS WEIGHT: 2.000 Date: 2008-12-11
MAIN-CHAIN ANGLE REFINED ATOMS COUNT: 2337 Date: 2008-12-11
MAIN-CHAIN ANGLE REFINED ATOMS RMS: 2.567 Date: 2008-12-11
MAIN-CHAIN ANGLE REFINED ATOMS WEIGHT: 4.000 Date: 2008-12-11
SIDE-CHAIN BOND REFINED ATOMS COUNT: 754 Date: 2008-12-11
SIDE-CHAIN BOND REFINED ATOMS RMS: 5.202 Date: 2008-12-11
SIDE-CHAIN BOND REFINED ATOMS WEIGHT: 6.000 Date: 2008-12-11
SIDE-CHAIN ANGLE REFINED ATOMS COUNT: 659 Date: 2008-12-11
SIDE-CHAIN ANGLE REFINED ATOMS RMS: 7.829 Date: 2008-12-11
SIDE-CHAIN ANGLE REFINED ATOMS WEIGHT: 8.000 Date: 2008-12-11
METHOD USED: BABINET MODEL WITH MASK Date: 2008-12-11
VDW PROBE RADIUS: 1.20 Date: 2008-12-11
ION PROBE RADIUS: 0.80 Date: 2008-12-11
SHRINKAGE RADIUS: 0.80 Date: 2008-12-11
Refinement Date : 2008-12-11 Date: 2008-12-11
Refinement Technical Note: qc_check1.html Date: 2008-12-11
Structure Refinement Summary: refine_summary1.txt Date: 2008-12-11
Matthews Coef: 4.32 Date: 2009-05-03
Solvent Percentage: 71.53 Date: 2009-05-03

Collection: 71498 Cell: 2 Date: 2009-01-29 Status: Success Previous Location: Collection: 71360 Cell: 2 Collection: 71357 Cell: 1 Collection: 71359 Cell: 1 Collection: 71358 Cell: 1 Collection: 71498 Cell: 1

Name of refinement run: ref2 Date: 2009-01-29
Performed By: 2793 Date: 2009-01-29
REFINEMENT TARGET :: MAXIMUM LIKELIHOOD WITH PHASES Date: 2009-01-29
RESOLUTION RANGE HIGH:: 2.20 Date: 2009-01-29
DATA CUTOFF (SIGMA(F)) :: NONE Date: 2009-01-29
COMPLETENESS (WORKING+TEST) (%) :: 99.94 Date: 2009-01-29
NUMBER OF REFLECTIONS :: 29154 Date: 2009-01-29
CROSS-VALIDATION METHOD :: THROUGHOUT Date: 2009-01-29
FREE R VALUE TEST SET SELECTION :: RANDOM Date: 2009-01-29
R VALUE (WORKING SET) :: 0.17003 Date: 2009-01-29
FREE R VALUE :: 0.20577 Date: 2009-01-29
FREE R VALUE TEST SET SIZE (%) :: 5.0 Date: 2009-01-29
FREE R VALUE TEST SET COUNT :: 1548 Date: 2009-01-29
TOTAL NUMBER OF BINS USED :: 20 Date: 2009-01-29
BIN RESOLUTION RANGE HIGH (A) :: 2.200 Date: 2009-01-29
BIN RESOLUTION RANGE LOW (A) :: 2.257 Date: 2009-01-29
REFLECTIONS IN BIN (WORKING SET) :: 2118 Date: 2009-01-29
BIN R VALUE (WORKING SET) :: 0.256 Date: 2009-01-29
BIN FREE R VALUE :: 0.286 Date: 2009-01-29
BIN FREE R VALUE TEST SET COUNT :: 116 Date: 2009-01-29
MEAN B VALUE (OVERALL, A**2) :: 34.533 Date: 2009-01-29
OVERALL ANISOTROPIC B VALUE B11 (A**2) :: 1.40 Date: 2009-01-29
OVERALL ANISOTROPIC B VALUE B22 (A**2) :: 1.40 Date: 2009-01-29
OVERALL ANISOTROPIC B VALUE B33 (A**2) :: -2.10 Date: 2009-01-29
OVERALL ANISOTROPIC B VALUE B12 (A**2) :: 0.70 Date: 2009-01-29
OVERALL ANISOTROPIC B VALUE B13 (A**2) :: 0.00 Date: 2009-01-29
OVERALL ANISOTROPIC B VALUE B23 (A**2) :: 0.00 Date: 2009-01-29
SPACE GROUP: P3221 Date: 2009-01-29
MAIN-CHAIN ANGLE RMS (A**2) :: 1.745 Date: 2009-01-29
BOND LENGTHS RMS DEVIATIONS (A) :: 0.016 Date: 2009-01-29
Refinement Log: submitted2.log Date: 2009-01-29
Reflection File: submitted2.mtz Date: 2009-01-29
PDB file: submitted2.pdb Date: 2009-01-29
Program: REFMAC Date: 2009-01-29
Version: 5.5.0053 Date: 2009-01-29
Cell A: 107.621 Date: 2009-01-29
Cell B: 107.621 Date: 2009-01-29
Cell C: 89.296 Date: 2009-01-29
Angle Alpha: 90.00 Date: 2009-01-29
Angle Beta: 90.00 Date: 2009-01-29
Angle Gamma: 120.00 Date: 2009-01-29
ALL ATOMS: 2459 Date: 2009-01-29
ESU BASED ON R VALUE: 0.136 Date: 2009-01-29
ESU BASED ON FREE R VALUE: 0.134 Date: 2009-01-29
ESU BASED ON MAXIMUM LIKELIHOOD: 0.092 Date: 2009-01-29
ESU FOR B VALUE BASED ON MAXIMUM LIKELIHOOD: 8.219 Date: 2009-01-29
CORRELATION COEFFICIENT FO-FC: 0.964 Date: 2009-01-29
CORRELATION COEFFICIENT FO-FC FREE: 0.946 Date: 2009-01-29
BOND LENGTHS REFINED ATOMS - LENGTH COUNT: 2281 Date: 2009-01-29
BOND LENGTHS REFINED ATOMS - LENGTH WEIGHT: 0.022 Date: 2009-01-29
BOND ANGLES REFINED ATOMS - ANGLE WEIGHT: 1.995 Date: 2009-01-29
CHIRAL-CENTER RESTRAINTS RMS: 0.102 Date: 2009-01-29
TORSION ANGLES, PERIOD COUNT: 297::83::366::1 Date: 2009-01-29
TORSION ANGLES, PERIOD RMS: 4.112::34.656::10.069::5.727 Date: 2009-01-29
TORSION ANGLES, PERIOD WEIGHT: 5.000::26.867::15.000::15.000 Date: 2009-01-29
CHIRAL-CENTER RESTRAINTS COUNT: 367 Date: 2009-01-29
CHIRAL-CENTER RESTRAINTS WEIGHT: 0.200 Date: 2009-01-29
GENERAL PLANES REFINED ATOMS COUNT: 2489 Date: 2009-01-29
GENERAL PLANES REFINED ATOMS RMS: 0.007 Date: 2009-01-29
GENERAL PLANES REFINED ATOMS WEIGHT: 0.021 Date: 2009-01-29
MAIN-CHAIN BOND REFINED ATOMS COUNT: 1460 Date: 2009-01-29
MAIN-CHAIN BOND REFINED ATOMS RMS: 1.148 Date: 2009-01-29
MAIN-CHAIN BOND REFINED ATOMS WEIGHT: 2.000 Date: 2009-01-29
MAIN-CHAIN ANGLE REFINED ATOMS COUNT: 2371 Date: 2009-01-29
MAIN-CHAIN ANGLE REFINED ATOMS RMS: 2.348 Date: 2009-01-29
MAIN-CHAIN ANGLE REFINED ATOMS WEIGHT: 4.000 Date: 2009-01-29
SIDE-CHAIN BOND REFINED ATOMS COUNT: 821 Date: 2009-01-29
SIDE-CHAIN BOND REFINED ATOMS RMS: 4.528 Date: 2009-01-29
SIDE-CHAIN BOND REFINED ATOMS WEIGHT: 6.000 Date: 2009-01-29
SIDE-CHAIN ANGLE REFINED ATOMS COUNT: 726 Date: 2009-01-29
SIDE-CHAIN ANGLE REFINED ATOMS RMS: 6.790 Date: 2009-01-29
SIDE-CHAIN ANGLE REFINED ATOMS WEIGHT: 8.000 Date: 2009-01-29
METHOD USED: BABINET MODEL WITH MASK Date: 2009-01-29
VDW PROBE RADIUS: 1.40 Date: 2009-01-29
ION PROBE RADIUS: 0.80 Date: 2009-01-29
SHRINKAGE RADIUS: 0.80 Date: 2009-01-29
Refinement Date : 2009-01-29 Date: 2009-01-29
Refinement Technical Note: qc_check2.html Date: 2009-01-29
Structure Refinement Summary: refine_summary2.txt Date: 2009-01-29
Matthews Coef: 4.32 Date: 2009-07-12
Solvent Percentage: 71.53 Date: 2009-07-12

STAGE: QC

Collection: 73001 Cell: 1 Date: 2009-01-28 Status: QC Return Previous Location: Collection: 71498 Cell: 1

QC Comments: See attached notes. Date: 2009-01-28
Performed By: 2015 Date: 2009-01-28
QC Log: qc_comments1.txt Date: 2009-01-28
QC Date: 2009-01-28 Date: 2009-01-28
QC Set Name: GS13738B_104883_qc1 Date: 2009-01-28

Collection: 73001 Cell: 2 Date: 2009-01-29 Status: Success Previous Location: Collection: 71498 Cell: 2

QC Comments: Ready for validation Date: 2009-01-29
Performed By: 2015 Date: 2009-01-29
QC Date: 2009-01-29 Date: 2009-01-29
QC Set Name: GS13783B_104883_qc2 Date: 2009-01-29

STAGE: Validation

Collection: 73081 Cell: 1 Date: 2009-02-02 Status: Success Previous Location: Collection: 73001 Cell: 2

STAGE: PDB Deposition

Collection: 73202 Cell: 1 Date: 2009-02-02 Status: Success Previous Location: Collection: 73081 Cell: 1

PDB ID: 3g3l Date: 2009-02-02
RCSB ID: RCSB051379 Date: 2009-02-02
PDB File: J--www-home-data-deposition_files-GS13738B-gs13738b.pdb Date: 2009-02-02
Coordinate mmCIF File: J--www-home-data-deposition_files-GS13738B-deposition-gs13738b_coor.mmCIF.gz Date: 2009-02-02
Structure factor mmCIF File: J--www-home-data-deposition_files-GS13738B-deposition-gs13738b_sf.mmCIF.gz Date: 2009-02-02
Deposition Date: 2009-02-02 Date: 2009-02-02


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