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Target ID: 355937
Target Name:SARS070  Protein Accession:29837500  Local Accession: SARS070  cDNA Accession:29837500  PSCA PDB:1ysyPDB:2kys

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Proposed Target  
 
Active Target  
 
PCR Testing  
 
Amplifer Generation  
 
Orientation  
 
Plasmid Purification  
 
Expression  
 
Protein Purification  
 
Crystal Setup  
 
Crystallization  
 
NMR Structure  
 
Refining  
 
Pre QC  
 
QC  
 
PDB Deposition  
 
Proposed Target
 
Active Target
 
PCR Testing
 
Amplifer Generation
 
Orientation
 
Plasmid Purification
 
Expression
 
Protein Purification
 
Crystal Setup
 
Crystallization
 
NMR Structure
 
Refining
 
Pre QC
 
QC
 
PDB Deposition
 
 
Status View
 
Chrono View
 
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Name: nonstructural Protein 7, Nsp7 replicase, PF08716 family  Start Position: 1  End Position: 83
GNF Target Alias: TSRI-SARS070-dum-83-1-83  Sequence Type: COMPLETE  Residues: 83
DNA Length: 249  Molecular Weight: 9267.33  Isoelectric Point: 5.18
Isoelectric Point (Incl. Tag): 6.35  Epsilon 280: 5500  Gravy Index: 0.19
Species: SARS-Tor2  Activated: 3  Alanine Absolute: 5
Alanine Relative: 0.0602  Arginine Absolute: 3  Arginine Relative: 0.0361
Asparagine Absolute: 3  Asparagine Relative: 0.0361  Aspartic Acid Absolute: 5
Aspartic Acid Relative: 0.0602  Cysteine Absolute: 3  Cysteine Relative: 0.0361
Glutamic Acid Absolute: 5  Glutamic Acid Relative: 0.0602  Glutamine Absolute: 6
Glutamine Relative: 0.0723  Glycine Absolute: 1  Glycine Relative: 0.0120
Histidine Absolute: 1  Histidine Relative: 0.0120  Isoleucine Absolute: 2
Isoleucine Relative: 0.0241  Leucine Absolute: 15  Leucine Relative: 0.1807
Lysine Absolute: 5  Lysine Relative: 0.0602  Methionine Absolute: 4
Methionine Relative: 0.0482  Phenylalanine Absolute: 1  Phenylalanine Relative: 0.0120
Proline Absolute: 0  Proline Relative: 0.0000  Serine Absolute: 10
Serine Relative: 0.1205  Threonine Absolute: 4  Threonine Relative: 0.0482
Tryptophan Absolute: 1  Tryptophan Relative: 0.0120  Tyrosine Absolute: 0
Tyrosine Relative: 0.0000  Valine Absolute: 9  Valine Relative: 0.1084
Project: SARS-1  Comment: No functional annotation in NCBI. The secondary structure prediction shows 4 consecutive helices, so the structure is almost surely a four helix bundle.  Selection Date: 2003-07-25
ProposalRelationshipOwnerLab Name
17JCSGTSRIThe Scripps Research Institute

STAGE: Proposed Target

STAGE: Active Target

STAGE: PCR Testing

STAGE: Amplifer Generation

STAGE: Orientation

STAGE: Plasmid Purification

STAGE: Expression

STAGE: Protein Purification

STAGE: Crystal Setup

STAGE: Crystallization

STAGE: NMR Structure

STAGE: Refining

STAGE: Pre QC

STAGE: QC

STAGE: PDB Deposition
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