JCSG Primer Selection Tool

Target List:
File Format: Gene Name <TAB> Start <TAB> End <NEWLINE>
Sequence FASTA file:
FASTA includes stop codons:
Temperature: C (62.5) Tolerance: C (2.5)
Salt Concentration: uM (100)
Tm = 81.5 + 16.6 log [Na+] + 41(G + C)/length - 500/length
Min Sequence: bp (18) Max Sequence: bp (35)
Theoretical bp Range:
Forward Primer Restriction Site:
Reverse Primer Restriction Site:
Prepend ATG to segments: Convert xTG to ATG on full or truncated sequences:
File Format
PCR Amplification Protocol
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